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Literature summary extracted from

  • Lazarus, M.; Levin, R.; Shokat, K.
    Discovery of new substrates of the elongation factor-2 kinase suggests a broader role in the cellular nutrient response (2017), Cell. Signal., 29, 78-83.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin required Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.20 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Rosetta 2 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.20 T482A catalytically inactive mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.20 EDTA
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ required Homo sapiens
2.7.11.20 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 additional information Homo sapiens mammalian enzyme elongation factor-2 kinase (eEF2K) a requirement for threonine residues as the target of phosphorylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens O00418
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein the enzyme performs autophosphorylation at Thr57 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.20 recombinant His-tagged enzyme from Escherichia coli strain BL21 Rosetta 2 by nickel affinity chromatography, ultrafiltration, cleavage of the tag with Sumo protease, and anion exchange chromatography, followed by gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + cortactin i.e. SRC8, an Src substrate, phosphorylation peptide sequence is EYQGK-T-EKHAS Homo sapiens ADP + phosphorylated cortactin
-
 ?
2.7.11.20 ATP + heat shock protein 60 i.e. HSPD1, phosphorylation peptide sequence is TKDGV-T-VAKSI Homo sapiens ADP + phosphorylated heat shock protein 60
-
 ?
2.7.11.20 ATP + hematological and neurological expressed 1-like protein i.e. HN1L, phosphorylation peptide sequence is FGSPV-T-ATSRL Homo sapiens ADP + phosphorylated hematological and neurological expressed 1-like protein
-
 ?
2.7.11.20 ATP + heterogeneous nuclear ribonucleoprotein A1 i.e. HNRNPA1, phosphorylation peptide sequence is VSRED-S-QRPGA Homo sapiens ADP + phosphorylated heterogeneous nuclear ribonucleoprotein A1
-
 ?
2.7.11.20 ATP + immunoglobulin-binding protein 1 i.e. IGBP1, phosphorylation peptide sequence is EDDEQ-T-LHRAR Homo sapiens ADP + phosphorylated immunoglobulin-binding protein 1
-
 ?
2.7.11.20 ATP + microtubule-associated protein 4 i.e. MAP4, phosphorylation peptide sequence is TEAAA-T-TRKPE Homo sapiens ADP + phosphorylated microtubule-associated protein 4
-
 ?
2.7.11.20 ATP + N-myc downstream-regulated gene 1 protein i.e. NDRG1, phosphorylation peptide sequence is TSLDG-T-RSRSH Homo sapiens ADP + phosphorylated N-myc downstream-regulated gene 1 protein
-
 ?
2.7.11.20 ATP + PEST proteolytic signal-containing nuclear protein i.e. PCNP, phosphorylation peptide sequence is AIGSQ-T-TKKAS Homo sapiens ADP + phosphorylated PEST proteolytic signal-containing nuclear protein
-
 ?
2.7.11.20 ATP + prothymosin alpha i.e. PTMA, phosphorylation peptide sequence is TSSEI-T-TKDLK Homo sapiens ADP + phosphorylated prothymosin alpha
-
 ?
2.7.11.20 ATP + SAP domain-containing ribonucleoprotein i.e. SARNP, phosphorylation peptide sequence is GTTED-T-EAKKR Homo sapiens ADP + phosphorylated SAP domain-containing ribonucleoprotein
-
 ?
2.7.11.20 ATP + scaffold attachment factor B2 i.e. SAFB2, phosphorylation peptide sequence is VISVK-T-TSRSK Homo sapiens ADP + phosphorylated scaffold attachment factor B2
-
 ?
2.7.11.20 ATP + U4/U6 small nuclear ribonucleoprotein Prp31 i.e. PRP31, phosphorylation peptide sequence is ERLGL-T-EIRKQ Homo sapiens ADP + phosphorylated U4/U6 small nuclear ribonucleoprotein Prp31
-
 ?
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 additional information mammalian enzyme elongation factor-2 kinase (eEF2K) a requirement for threonine residues as the target of phosphorylation Homo sapiens ?
-
 ?
2.7.11.20 additional information the enzyme performs autophosphorylation at Thr57. Additional enzyme protein substrates show a strong sequence motif consisting of acidic residues in the -2 position and basic residues in the +3 position and almost exclusively threonine as the phosphorylated residue, peptide mapping and mass spectrometry analysis, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 eEF2 kinase
-
 Homo sapiens
2.7.11.20 eEF2K
-
 Homo sapiens
2.7.11.20 elongation factor-2 kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.20 22
-
 assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.20 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.20 ATP
-
 Homo sapiens
2.7.11.20 ATPgammaS i.e. ATP-gamma-thiophosphate Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.11.20 evolution eEF2K is a calcium/calmodulin dependent kinase that belongs to the alpha kinase family, which has negligible sequence identity to the conventional protein kinase family Homo sapiens
2.7.11.20 additional information homology modelling of eEF2K using the structure of myosin heavy chain kinase, EC 2.7.11.7, PDB ID 3LMH. A glutamate might act as gatekeeper and makes likely hydrogen bonds to the adenine in the active site and is essential for activity in the alpha kinase family Homo sapiens
2.7.11.20 physiological function the phosphorylation of eEF2 through eEF2 kinase blocks translation elongation, which is thought to be critical to regulating cellular energy usage. eEF2K has a role in regulating cellular energy usage that involves multiple pathways and regulatory feedback Homo sapiens