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Literature summary extracted from

  • Ming, Z.; Wang, W.; Xie, Y.; Ding, P.; Chen, Y.; Jin, D.; Sun, Y.; Xia, B.; Yan, L.; Lou, Z.
    Crystal structure of the novel di-nucleotide cyclase from Vibrio cholerae (DncV) responsible for synthesizing a hybrid cyclic GMP-AMP (2014), Cell Res., 24, 1270-1273.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.B24 additional information no activitation by binding to DNa required Vibrio cholerae O1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.B24 wild-type and mutant D131N/D133N. Catalytic residues are D131 and D133 in strand beta1, and D193 in strand beta5, and the G[G/S]xx motif is defined by residues G113-S114-F115-Q116 in a well-defined loop linking helix alpha5 and strand beta1 Vibrio cholerae O1

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.B24 D131N mutation in conserved acidic residue, loss of activity Vibrio cholerae O1
2.7.7.B24 D131N/D133N mutation in conserved acidic residues, loss of activity Vibrio cholerae O1
2.7.7.B24 D133N mutation in conserved acidic residue, loss of activity Vibrio cholerae O1
2.7.7.B24 D193N mutation in conserved acidic residue, loss of activity Vibrio cholerae O1
2.7.7.B24 K287A mutation in residue that participates in the interaction with diphosphate, impairs the ability to catalyze cyclic di-GMP formation Vibrio cholerae O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.B24 ATP + GTP Vibrio cholerae O1
-
 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
2.7.7.B24 ATP + GTP Vibrio cholerae O1 ATCC 39315
-
 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
2.7.7.B24 GTP + ATP Vibrio cholerae O1
-
 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
2.7.7.B24 GTP + ATP Vibrio cholerae O1 ATCC 39315
-
 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
2.7.7.B24 GTP + GTP Vibrio cholerae O1
-
 2 diphosphate + cyclic Gp(3'-5')G
-
 ?
2.7.7.B24 GTP + GTP Vibrio cholerae O1 ATCC 39315
-
 2 diphosphate + cyclic Gp(3'-5')G
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B24 Vibrio cholerae O1 Q9KVG7
-
-
2.7.7.B24 Vibrio cholerae O1 ATCC 39315 Q9KVG7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B24 ATP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
2.7.7.B24 ATP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Ap(3'-5')Gp a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated ?
2.7.7.B24 ATP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
2.7.7.B24 ATP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Ap(3'-5')Gp a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated ?
2.7.7.B24 GTP + ATP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
2.7.7.B24 GTP + ATP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
2.7.7.B24 GTP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Gp(3'-5')G
-
 ?
2.7.7.B24 GTP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Gp(3'-5')G
-
 ?