Crystal structure of the novel di-nucleotide cyclase from Vibrio cholerae (DncV) responsible for synthesizing a hybrid cyclic GMP-AMP
Ming, Z.; Wang, W.; Xie, Y.; Ding, P.; Chen, Y.; Jin, D.; Sun, Y.; Xia, B.; Yan, L.; Lou, Z.; Cell Res. 24, 1270-1273 (2014) 
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.B24
additional information
no activitation by binding to DNa required
Vibrio cholerae O1
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
2.7.7.B24
wild-type and mutant D131N/D133N. Catalytic residues are D131 and D133 in strand beta1, and D193 in strand beta5, and the G[G/S]xx motif is defined by residues G113-S114-F115-Q116 in a well-defined loop linking helix alpha5 and strand beta1
Vibrio cholerae O1
Engineering
EC Number
Protein Variants
Commentary
Organism
2.7.7.B24
D131N
mutation in conserved acidic residue, loss of activity
Vibrio cholerae O1
2.7.7.B24
D131N/D133N
mutation in conserved acidic residues, loss of activity
Vibrio cholerae O1
2.7.7.B24
D133N
mutation in conserved acidic residue, loss of activity
Vibrio cholerae O1
2.7.7.B24
D193N
mutation in conserved acidic residue, loss of activity
Vibrio cholerae O1
2.7.7.B24
K287A
mutation in residue that participates in the interaction with diphosphate, impairs the ability to catalyze cyclic di-GMP formation
Vibrio cholerae O1
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.7.B24
ATP + GTP
Vibrio cholerae O1
-
2 diphosphate + cyclic Ap(3'-5')G
-
-
?
2.7.7.B24
ATP + GTP
Vibrio cholerae O1 ATCC 39315
-
2 diphosphate + cyclic Ap(3'-5')G
-
-
?
2.7.7.B24
GTP + ATP
Vibrio cholerae O1
-
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
?
2.7.7.B24
GTP + ATP
Vibrio cholerae O1 ATCC 39315
-
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
?
2.7.7.B24
GTP + GTP
Vibrio cholerae O1
-
2 diphosphate + cyclic Gp(3'-5')G
-
-
?
2.7.7.B24
GTP + GTP
Vibrio cholerae O1 ATCC 39315
-
2 diphosphate + cyclic Gp(3'-5')G
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.7.B24
Vibrio cholerae O1
Q9KVG7
-
-
2.7.7.B24
Vibrio cholerae O1 ATCC 39315
Q9KVG7
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Ap(3'-5')G
a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated
-
-
?
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Ap(3'-5')G
a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated
-
-
?
2.7.7.B24
GTP + ATP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
-
?
2.7.7.B24
GTP + ATP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
-
?
2.7.7.B24
GTP + GTP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
2.7.7.B24
GTP + GTP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.B24
additional information
no activitation by binding to DNa required
Vibrio cholerae O1
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.7.B24
wild-type and mutant D131N/D133N. Catalytic residues are D131 and D133 in strand beta1, and D193 in strand beta5, and the G[G/S]xx motif is defined by residues G113-S114-F115-Q116 in a well-defined loop linking helix alpha5 and strand beta1
Vibrio cholerae O1
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.7.7.B24
D131N
mutation in conserved acidic residue, loss of activity
Vibrio cholerae O1
2.7.7.B24
D131N/D133N
mutation in conserved acidic residues, loss of activity
Vibrio cholerae O1
2.7.7.B24
D133N
mutation in conserved acidic residue, loss of activity
Vibrio cholerae O1
2.7.7.B24
D193N
mutation in conserved acidic residue, loss of activity
Vibrio cholerae O1
2.7.7.B24
K287A
mutation in residue that participates in the interaction with diphosphate, impairs the ability to catalyze cyclic di-GMP formation
Vibrio cholerae O1
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.7.B24
ATP + GTP
Vibrio cholerae O1
-
2 diphosphate + cyclic Ap(3'-5')G
-
-
?
2.7.7.B24
ATP + GTP
Vibrio cholerae O1 ATCC 39315
-
2 diphosphate + cyclic Ap(3'-5')G
-
-
?
2.7.7.B24
GTP + ATP
Vibrio cholerae O1
-
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
?
2.7.7.B24
GTP + ATP
Vibrio cholerae O1 ATCC 39315
-
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
?
2.7.7.B24
GTP + GTP
Vibrio cholerae O1
-
2 diphosphate + cyclic Gp(3'-5')G
-
-
?
2.7.7.B24
GTP + GTP
Vibrio cholerae O1 ATCC 39315
-
2 diphosphate + cyclic Gp(3'-5')G
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Ap(3'-5')G
a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated
-
-
?
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
ATP + GTP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Ap(3'-5')G
a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated
-
-
?
2.7.7.B24
GTP + ATP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
-
?
2.7.7.B24
GTP + ATP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')A
in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated
-
-
?
2.7.7.B24
GTP + GTP
-
740274
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
2.7.7.B24
GTP + GTP
-
740274
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?