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Literature summary extracted from

  • Alcala, D.B.; Haldeman, B.D.; Brizendine, R.K.; Krenc, A.K.; Baker, J.E.; Rock, R.S.; Cremo, C.R.
    Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates (2016), Cell Biochem. Funct., 34, 469-474.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 Calmodulin dependent on Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 additional information MLCK is inhibited as the ionic strength increases Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 additional information
-
 additional information steady-state kinetic parameters of MLCK toward 4 different substrates: the 2-headed heavy meromyosin (HMM) soluble subfragments of both smooth muscle myosin II and non-muscle myosin IIB, each containing their respective smooth or non-muscle regulatory light chains, and the 2 types of free regulatory light chains. MLCK has an about 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar Gallus gallus
2.7.11.18 0.0025
-
 [non-muscle heavy meromyosin IIB] pH 7.6, 25°C Gallus gallus
2.7.11.18 0.0026
-
 [smooth muscle heavy meromyosin] pH 7.6, 25°C Gallus gallus
2.7.11.18 0.0051
-
 [non-muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
2.7.11.18 0.0072
-
 [smooth muscle regulatory light chain] pH 7.6, 25°C Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ dependent on Gallus gallus
2.7.11.18 Mg2+ required Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Gallus gallus non-muscle myosin II and preferred substrate smooth muscle myosin II ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 additional information Gallus gallus non-muscle myosin and smooth muscle myosin are the only known substrates of MLCK in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Gallus gallus P11799
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 gizzard
-
 Gallus gallus
-
2.7.11.18 additional information myosin light chain kinase is a ubiquitous Ca2+/calmodulin (CaM)-activated kinase found in smooth, cardiac, and skeletal muscle as well as in mammalian non-muscle cells Gallus gallus
-
2.7.11.18 smooth muscle
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] non-muscle myosin II and preferred substrate smooth muscle myosin II Gallus gallus ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] phosphorylation of myosin on Ser19 of the regulatory light chain. Non-muscle myosin II regulatory light chain and preferred substrate smooth muscle myosin II regulatory light chain Gallus gallus ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [non-muscle heavy meromyosin IIB]
-
 Gallus gallus ADP + [non-muscle heavy meromyosin IIB] phosphate
-
 ?
2.7.11.18 ATP + [non-muscle regulatory light chain]
-
 Gallus gallus ADP + [non-muscle regulatory light chain] phosphate
-
 ?
2.7.11.18 ATP + [smooth muscle heavy meromyosin]
-
 Gallus gallus ADP + [smooth muscle heavy meromyosin] phosphate
-
 ?
2.7.11.18 ATP + [smooth muscle regulatory light chain]
-
 Gallus gallus ADP + [smooth muscle regulatory light chain] phosphate
-
 ?
2.7.11.18 additional information non-muscle myosin and smooth muscle myosin are the only known substrates of MLCK in vivo Gallus gallus ?
-
 ?
2.7.11.18 additional information MLCK has a 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar. Myosin light chain kinase has a 1.6fold and 1.5fold higher specificity (kcat/Km) for smooth versus non-muscle-free regulatory light chain and heavy meromyosin, respectively, suggesting that differences in specificity are dictated by regulatory light chain sequences. Three residues, Glu70, Gly71, and Ser74, are found to be surface exposed in the N-terminal half of the regulatory light chain, consistent with their importance in substrate recognition. Substrate recognition and specificity, overview Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Gallus gallus
2.7.11.18 MLCK-108
-
 Gallus gallus
2.7.11.18 MYLK
-
 Gallus gallus
2.7.11.18 myosin light chain kinase
-
 Gallus gallus
2.7.11.18 smooth muscle MLCK
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 25
-
 assay at Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.18 additional information
-
 additional information steady-state kinetics: comparison of smooth muscle myosin II and non-muscle myosin IIB as substrates, overview. MLCK has an about 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar Gallus gallus
2.7.11.18 8
-
 [non-muscle heavy meromyosin IIB] pH 7.6, 25°C Gallus gallus
2.7.11.18 12.6
-
 [non-muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
2.7.11.18 12.8
-
 [smooth muscle heavy meromyosin] pH 7.6, 25°C Gallus gallus
2.7.11.18 28.6
-
 [smooth muscle regulatory light chain] pH 7.6, 25°C Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.18 7.6
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Gallus gallus

General Information

EC Number General Information Comment Organism
2.7.11.18 malfunction in knockout mice, the lack of MLCK-mediated phosphorylation of myosin in tracheal, bronchial, and gastrointestinal smooth muscle cannot be rescued by other kinases Gallus gallus
2.7.11.18 physiological function phosphorylation of myosin on S19 of the regulatory light chain (also called LC20) activates myosin ATPase activity in the presence of actin, which is necessary and sufficient for muscle contraction. All smooth muscles express two forms of myosin II, SMM (most abundant in adult tissues; includes 4 alternatively spliced variants derived from a single SMM heavy chain gene: 1A, 1B, 2A, and 2B) and NMM (IIA, IIB, and IIC minor). The smooth muscle enzyme prefers the smooth muscle myosin II regulatory light chain to the non-muscle myosin II. Non-muscle myosin IIB may participate in a physiologically relevant pathway for force maintenance in smooth muscle Gallus gallus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.11.18 additional information
-
 additional information myosin light chain kinase has a 1.6fold and 1.5fold higher kcat/Km for smooth versus non-muscle-free regulatory light chain and heavy meromyosin, respectively Gallus gallus
2.7.11.18 2470
-
 [non-muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
2.7.11.18 3200
-
 [non-muscle heavy meromyosin IIB] pH 7.6, 25°C Gallus gallus
2.7.11.18 3972
-
 [smooth muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
2.7.11.18 4923
-
 [smooth muscle heavy meromyosin] pH 7.6, 25°C Gallus gallus