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Literature summary extracted from

  • Zhang, P.; Shao, Z.; Jin, W.; Duan, D.
    Comparative characterization of two GDP-mannose dehydrogenase genes from Saccharina japonica (Laminariales, Phaeophyceae) (2016), BMC Plant Biol., 16, 62.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.132 expressed in Escherichia coli Saccharina japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.132 Ca2+ inhibitor of isoform GMD2 with 71.86% residual activity at 1 mM Saccharina japonica
1.1.1.132 Mn2+
-
 Saccharina japonica
1.1.1.132 Zn2+ potent inhibitor with 0.75% residual activity at 1 mM for both isoforms GMD1 and GMD2 Saccharina japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.132 0.139
-
 NAD+ isoform GMD1, at pH 8.0 and 30°C Saccharina japonica
1.1.1.132 0.177
-
 GDP-D-mannose isoform GMD2, at pH 8.3 and 20°C Saccharina japonica
1.1.1.132 0.195
-
 NAD+ isoform GMD2, at pH 8.3 and 20°C Saccharina japonica
1.1.1.132 0.289
-
 GDP-D-mannose isoform GMD1, at pH 8.0 and 30°C Saccharina japonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.132 Ca2+ activator of isoform GMD1 with 178.05% activity at 1 mM Saccharina japonica
1.1.1.132 Mg2+ 133.79% activity of isoform GMD1 at 1 mM, 120.25% activity of isoform GMD2 at 1 mM Saccharina japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.132 34460
-
-
 Saccharina japonica
1.1.1.132 35200
-
-
 Saccharina japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.132 GDP-D-mannose + NAD+ + H2O Saccharina japonica
-
 GDP-D-mannuronate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.132 Saccharina japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.132 amylose resin column chromatography Saccharina japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.132 GDP-D-mannose + NAD+ + H2O
-
 Saccharina japonica GDP-D-mannuronate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.132 ? x * 34460, isoform GMD2, calculated from amino acid sequence Saccharina japonica
1.1.1.132 ? x * 35200, isoform GMD2, calculated from amino acid sequence Saccharina japonica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.132 GDP-mannose dehydrogenase
-
 Saccharina japonica
1.1.1.132 GMD1 isoform Saccharina japonica
1.1.1.132 GMD2 isoform Saccharina japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.132 20
-
 isoform GMD1 Saccharina japonica
1.1.1.132 30
-
 isoform GMD1 Saccharina japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.132 8
-
 isoform GMD1 Saccharina japonica
1.1.1.132 8.3
-
 isoform GMD2 Saccharina japonica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.132 NAD+
-
 Saccharina japonica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.132 Saccharina japonica isoform GMD1, calculated from amino acid sequence
-
 5.2
1.1.1.132 Saccharina japonica isoform GMD2, calculated from amino acid sequence
-
 5.4