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Literature summary extracted from

  • Gao, L.; Du, G.; Zhou, J.; Chen, J.; Liu, J.
    Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-keto-L-gulonic acid in Ketogulonicigenium vulgare WSH-001 (2013), Biotechnol. Prog., 29, 1398-1404.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.32 expressed in Escherichia coli BL21(DE3) cells Ketogulonicigenium vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.32 L-sorbose + 2,6-dichlorophenolindophenol Ketogulonicigenium vulgare
-
 L-sorbosone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 L-sorbose + 2,6-dichlorophenolindophenol Ketogulonicigenium vulgare WSH-001
-
 L-sorbosone + reduced 2,6-dichlorophenolindophenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.32 Ketogulonicigenium vulgare
-
-
-
1.1.99.32 Ketogulonicigenium vulgare WSH-001
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.32 HisTrap column chromatography, and Superdex 200 gel filtration Ketogulonicigenium vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.32 L-sorbose + 2,6-dichlorophenolindophenol
-
 Ketogulonicigenium vulgare L-sorbosone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 L-sorbose + 2,6-dichlorophenolindophenol
-
 Ketogulonicigenium vulgare WSH-001 L-sorbosone + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.32 PQQ-dependent L-sorbose dehydrogenase
-
 Ketogulonicigenium vulgare
1.1.99.32 SDH
-
 Ketogulonicigenium vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.32 pyrroloquinoline quinone
-
 Ketogulonicigenium vulgare