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Literature summary extracted from

  • Han, X.; Xiong, X.; Jiang, D.; Chen, S.; Huang, E.; Zhang, W.; Liu, X.
    Crystal structure of L-sorbose dehydrogenase, a pyrroloquinoline quinone-dependent enzyme with homodimeric assembly, from Ketogulonicigenium vulgare (2014), Biotechnol. Lett., 36, 1001-1008.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.32 expressed in Escherichia coli codon plus cells Ketogulonicigenium vulgare

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.32 hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 5 mM pyrroloquinoline quinone, 5 mM CaCl2, and 0.1 M pH 7.4 HEPES Ketogulonicigenium vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.32 membrane
-
 Ketogulonicigenium vulgare 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.32 Ca2+ required Ketogulonicigenium vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.32 117200
-
 calculated from amino acid sequence Ketogulonicigenium vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.32 L-sorbose + cytochrome c551 Ketogulonicigenium vulgare
-
 L-sorbosone + reduced cytochrome c551
-
 ?
1.1.99.32 L-sorbose + cytochrome c551 Ketogulonicigenium vulgare Y25
-
 L-sorbosone + reduced cytochrome c551
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.32 Ketogulonicigenium vulgare
-
-
-
1.1.99.32 Ketogulonicigenium vulgare Y25
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.32 Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration Ketogulonicigenium vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.32 L-sorbose + cytochrome c551
-
 Ketogulonicigenium vulgare L-sorbosone + reduced cytochrome c551
-
 ?
1.1.99.32 L-sorbose + cytochrome c551
-
 Ketogulonicigenium vulgare Y25 L-sorbosone + reduced cytochrome c551
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.32 homodimer
-
 Ketogulonicigenium vulgare

Synonyms

EC Number Synonyms Comment Organism
1.1.99.32 L-sorbose dehydrogenase
-
 Ketogulonicigenium vulgare
1.1.99.32 SDH
-
 Ketogulonicigenium vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.32 pyrroloquinoline quinone
-
 Ketogulonicigenium vulgare