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Literature summary extracted from

  • Lama, S.; Ro, S.; Seol, E.; Sekar, B.; Ainala, S.; Thangappan, J.; Song, H.; Seung, D.; Park, S.
    Characterization of 1,3-propanediol oxidoreductase (DhaT) from Klebsiella pneumoniae J2B (2015), Biotechnol. Bioprocess Eng., 20, 971-979.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.202 additional information poor effects by EDTA, 2-mercaptoethanol, and sodium carbazide Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in YqhD-deficient Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.202 Ag+ complete inhibition at 1 mM Klebsiella pneumoniae
1.1.1.202 Co2+ 29% inhibition at 1 mM Klebsiella pneumoniae
1.1.1.202 Cu2+ 47% inhibition at 1 mM Klebsiella pneumoniae
1.1.1.202 Hg2+ complete inhibition at 1 mM Klebsiella pneumoniae
1.1.1.202 KCl 17% inhibition at 1 mM Klebsiella pneumoniae
1.1.1.202 additional information poor effects by LiCl, DTT and sodium bisulfite at 1 mM Klebsiella pneumoniae
1.1.1.202 Ni2+ 29% inhibition at 1 mM Klebsiella pneumoniae
1.1.1.202 Zn2+ 38% inhibition at 1 mM Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.202 0.03
-
 NADH pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 0.23
-
 NAD+ pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 0.77
-
 3-hydroxypropanal pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 0.94
-
 propionaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 2.16
-
 Valeraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 4.2
-
 Butyraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 4.25
-
 acetaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 7.44
-
 Propane-1,3-diol pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 16.1
-
 formaldehyde pH 7.0, 37°C Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.202 additional information no effects by NaCl, NH4Cl, and MgCl2 at 1 mM Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+ Klebsiella pneumoniae under physiological conditions, DhaT mostly catalyzes the forward reaction propane-1,3-diol + NAD+
-
 r
1.1.1.202 3-hydroxypropanal + NADH + H+ Klebsiella pneumoniae J2B under physiological conditions, DhaT mostly catalyzes the forward reaction propane-1,3-diol + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.202 Klebsiella pneumoniae Q59477
-
-
1.1.1.202 Klebsiella pneumoniae J2B Q59477
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.202 soluble recombinant His-tagged enzyme 6.6-6.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.202 1.9
-
 crude cell extract with recombinant His-tagged enzyme, substrate propane-1,3-diol, pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 13.1
-
 purified recombinant His-tagged enzyme, substrate propane-1,3-diol, pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 13.7
-
 crude cell extract with recombinant His-tagged enzyme, substrate 3-hydroxypropanal, pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 89.7
-
 purified recombinant His-tagged enzyme, substrate 3-hydroxypropanal, pH 7.0, 37°C Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r
1.1.1.202 3-hydroxypropanal + NADH + H+ under physiological conditions, DhaT mostly catalyzes the forward reaction Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r
1.1.1.202 3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae J2B propane-1,3-diol + NAD+
-
 r
1.1.1.202 3-hydroxypropanal + NADH + H+ under physiological conditions, DhaT mostly catalyzes the forward reaction Klebsiella pneumoniae J2B propane-1,3-diol + NAD+
-
 r
1.1.1.202 acetaldehyde + NADH + H+
-
 Klebsiella pneumoniae ethanol + NAD+
-
 ?
1.1.1.202 acetaldehyde + NADH + H+
-
 Klebsiella pneumoniae J2B ethanol + NAD+
-
 ?
1.1.1.202 butyraldehyde + NADH + H+
-
 Klebsiella pneumoniae butan-1-ol + NAD+
-
 ?
1.1.1.202 formaldehyde + NADH + H+
-
 Klebsiella pneumoniae methanol + NAD+
-
 ?
1.1.1.202 formaldehyde + NADH + H+
-
 Klebsiella pneumoniae J2B methanol + NAD+
-
 ?
1.1.1.202 propane-1,3-diol + NAD+
-
 Klebsiella pneumoniae 3-hydroxypropanal + NADH + H+
-
 r
1.1.1.202 propane-1,3-diol + NAD+
-
 Klebsiella pneumoniae J2B 3-hydroxypropanal + NADH + H+
-
 r
1.1.1.202 propionaldehyde + NADH + H+
-
 Klebsiella pneumoniae propan-1-ol + NAD+
-
 ?
1.1.1.202 valeraldehyde + NADH + H+
-
 Klebsiella pneumoniae pentan-1-ol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.202 1,3-propanediol oxidoreductase
-
 Klebsiella pneumoniae
1.1.1.202 DhaT
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.202 37
-
 aldehyde reduction Klebsiella pneumoniae
1.1.1.202 55
-
 alcohol oxidation Klebsiella pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.202 30 55 over 80% of maximal activity within this range, aldehyde reduction, profile overview Klebsiella pneumoniae
1.1.1.202 30 65 over 60% of maximal activity within this range, alcohol oxidation, profile overview Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.202 1
-
 Valeraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 1.1
-
 propionaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 1.6
-
 formaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 5.2
-
 acetaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 8.7
-
 Butyraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 9.7
-
 NAD+ pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 9.98
-
 Propane-1,3-diol pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 59.4
-
 3-hydroxypropanal pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 59.5
-
 NADH pH 7.0, 37°C Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.202 7
-
 aldehyde reduction Klebsiella pneumoniae
1.1.1.202 9
-
 alcohol oxidation Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.202 5 9.5 activity range, aldehyde reduction, profile overview Klebsiella pneumoniae
1.1.1.202 8 10 activity range, alcohol oxidation, profile overview Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.202 NAD+
-
 Klebsiella pneumoniae
1.1.1.202 NADH
-
 Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
1.1.1.202 additional information enzyme homology modeling and docking studies Klebsiella pneumoniae
1.1.1.202 physiological function Klebsiella pneumoniae converts 3 hydroxypropionaldehyde (3-HPA) to 1,3-propanediol (1,3-PD) during microbial production of 1,3-PD from glycerol Klebsiella pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.202 0.1
-
 formaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 0.5
-
 Valeraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 1.1
-
 propionaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 1.1
-
 Propane-1,3-diol pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 1.2
-
 acetaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 2.1
-
 Butyraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 42
-
 NAD+ pH 9.0, 55°C Klebsiella pneumoniae
1.1.1.202 76.6
-
 3-hydroxypropanal pH 7.0, 37°C Klebsiella pneumoniae
1.1.1.202 1723
-
 NADH pH 7.0, 37°C Klebsiella pneumoniae