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Literature summary extracted from

  • Frommhagen, M.; Koetsier, M.J.; Westphal, A.H.; Visser, J.; Hinz, S.W.; Vincken, J.P.; van Berkel, W.J.; Kabel, M.A.; Gruppen, H.
    Lytic polysaccharide monooxygenases from Myceliophthora thermophila C1 differ in substrate preference and reducing agent specificity (2016), Biotechnol. Biofuels, 9, 186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.54
-
 Thermothelomyces thermophilus
1.14.99.56
-
 Thermothelomyces thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.54 comparison of isoforms LPMO9A, LPMO9B and LPMO9C. LPMO9B contains distal from the coordinated copper sphere an additional loop (Gly115-Asn121), which is not present in LPMO9A and LPMO9C. The copper ion in LPMO9A, LPMO9B and LPMO9C is coordinated by His1-His68-Tyr153, His1-His79-Tyr170 and His1-His84-Tyr166, respectively. All three LPMOs share two putative disulfide bridges Thermothelomyces thermophilus
1.14.99.56 comparison of isoforms LPMO9A, LPMO9B and LPMO9C. LPMO9B contains distal from the coordinated copper sphere an additional loop (Gly115-Asn121), which is not present in LPMO9A and LPMO9C. The copper ion in LPMO9A, LPMO9B and LPMO9C is coordinated by His1-His68-Tyr153, His1-His79-Tyr170 and His1-His84-Tyr166, respectively. All three LPMOs share two putative disulfide bridges Thermothelomyces thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.54 Cu2+
-
 Thermothelomyces thermophilus
1.14.99.56 Cu2+
-
 Thermothelomyces thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.54 32000
-
-
 Thermothelomyces thermophilus
1.14.99.56 25000
-
-
 Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Thermothelomyces thermophilus A0A0H4K9X4
-
-
1.14.99.54 Thermothelomyces thermophilus A0A1C9CXI1
-
-
1.14.99.56 Thermothelomyces thermophilus A0A1C9CXI0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 beta-(1->3,1->4)-glucan + acceptor + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized oxidized glucan oligosaccharides + reduced acceptor + H2O
-
 ?
1.14.99.54 cellulose + ascorbate + O2 substrate is regenerated amorphous cellulose Thermothelomyces thermophilus C1-oxidized cellooligosaccharides + cellooligosaccharides + dehydroascorbate + H2O release of C1-oxidized and non-oxidized glucooligosaccharides ?
1.14.99.54 cellulose + ascorbate + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.54 cellulose + oxidized dopamine + O2
-
 Thermothelomyces thermophilus C1-oxidized gluco-oligosaccharides + glucooligosaccharides + dopamine + H2O dopamine shows 46% of the activity with ascorbate ?
1.14.99.54 additional information for isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give the highest formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid Thermothelomyces thermophilus ?
-
 ?
1.14.99.54 additional information no substrate: beta-(1->3, 1->4)-glucan. For isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give the highest formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid Thermothelomyces thermophilus ?
-
 ?
1.14.99.54 xylan + dopamine + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized oxidized xylo-oligosaccharides + 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O 93% of the activiy with ascorbate ?
1.14.99.54 xyloglucan + acceptor + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized oxidized oligosaccharides + reduced acceptor + H2O
-
 ?
1.14.99.56 beta-(1->3,1->4)-glucan + ascorbate + O2 substrate is regenerated amorphous cellulose Thermothelomyces thermophilus C4-oxidized glucan oligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.56 cellulose + ascorbate + O2 substrate is regenerated amorphous cellulose Thermothelomyces thermophilus C4-oxidized gluco-oligosaccharides + dehydroascorbate + H2O sole release of C4-oxidized and non-oxidized gluco-oligosaccharides ?
1.14.99.56 cellulose + dopamine + O2
-
 Thermothelomyces thermophilus C4-oxidized gluco-oligosaccharides + 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O dopamine shows 6% of the activity with ascorbate ?
1.14.99.56 additional information for isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid Thermothelomyces thermophilus ?
-
 ?
1.14.99.56 xyloglucan + ascorbate + O2
-
 Thermothelomyces thermophilus C4-oxidized oligosaccharides + dehydroascorbate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.54 ? x * 30633, calculated Thermothelomyces thermophilus
1.14.99.56 ? x * 23449, calculated Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 LPMO9A
-
 Thermothelomyces thermophilus
1.14.99.54 LPMO9B
-
 Thermothelomyces thermophilus
1.14.99.56 LPMO9C
-
 Thermothelomyces thermophilus