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Literature summary extracted from

  • Fanuel, M.; Garajova, S.; Ropartz, D.; McGregor, N.; Brumer, H.; Rogniaux, H.; Berrin, J.G.
    The Podospora anserina lytic polysaccharide monooxygenase PaLPMO9H catalyzes oxidative cleavage of diverse plant cell wall matrix glycans (2017), Biotechnol. Biofuels, 10, 63.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.B10 expression in Pichia pastoris Podospora anserina

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.B10 Podospora anserina B2ADG1
-
-
1.14.99.B10 Podospora anserina DSM 980 B2ADG1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.B10 barley mixed-linkage beta-glucan + ascorbate + O2
-
 Podospora anserina ? + dehydroascorbate + H2O most abundant products show a degree of polymerization of 2-4 ?
1.14.99.B10 barley mixed-linkage beta-glucan + ascorbate + O2
-
 Podospora anserina DSM 980 ? + dehydroascorbate + H2O most abundant products show a degree of polymerization of 2-4 ?
1.14.99.B10 cellulose + ascorbate + O2
-
 Podospora anserina cellooligosaccharides + dehydroascorbate + H2O cleavage generates singly and doubly oxidized products at the C1 and/or C4 positions ?
1.14.99.B10 cellulose + ascorbate + O2
-
 Podospora anserina DSM 980 cellooligosaccharides + dehydroascorbate + H2O cleavage generates singly and doubly oxidized products at the C1 and/or C4 positions ?
1.14.99.B10 konjac glucomannan + ascorbate + O2
-
 Podospora anserina cellooligosaccharides + dehydroascorbate + H2O products are aldonic acids and gem-diols ?
1.14.99.B10 konjac glucomannan + ascorbate + O2
-
 Podospora anserina DSM 980 cellooligosaccharides + dehydroascorbate + H2O products are aldonic acids and gem-diols ?
1.14.99.B10 lichenan + ascorbate + O2
-
 Podospora anserina ? + dehydroascorbate + H2O most abundant products show a degree of polymerization of 2-4 ?
1.14.99.B10 lichenan + ascorbate + O2
-
 Podospora anserina DSM 980 ? + dehydroascorbate + H2O most abundant products show a degree of polymerization of 2-4 ?
1.14.99.B10 additional information LPMO9H is able to target polysaccharides differing from cellulose by their linkage types, glycosidic composition or the presence of sidechains. Isoform LPMO9H catalyzes C4 oxidative cleavage of mixed-linkage glucans, and mixed C1/C4 oxidative cleavage of glucomannan and xyloglucan. Gem-diols and ketones are produced at the non-reducing end, while aldonic acids are produced at the reducing extremity of the products Podospora anserina ?
-
 ?
1.14.99.B10 additional information LPMO9H is able to target polysaccharides differing from cellulose by their linkage types, glycosidic composition or the presence of sidechains. Isoform LPMO9H catalyzes C4 oxidative cleavage of mixed-linkage glucans, and mixed C1/C4 oxidative cleavage of glucomannan and xyloglucan. Gem-diols and ketones are produced at the non-reducing end, while aldonic acids are produced at the reducing extremity of the products Podospora anserina DSM 980 ?
-
 ?
1.14.99.B10 tamarind xyloglucan + ascorbate + O2
-
 Podospora anserina xylooligosaccharides + dehydroascorbate + H2O products are mostly oligosaccharides having degrees of polymerization of 6 and 7 ?
1.14.99.B10 XXXGXXXG + ascorbate + O2 branched substrate, G represents an unbranched beta-(1->4)-linked glucosyl residue and X represents beta-(1->4)-linked Glc bearing an alpha-(1->6)-linked xylosyl branch Podospora anserina ? + dehydroascorbate + H2O cleavage generates a gem-diol at the non-reducing end ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.B10 Lpmo9H
-
 Podospora anserina
1.14.99.B10 PODANS_4_1020
-
 Podospora anserina