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Literature summary extracted from

  • Kulig, J.; Frese, A.; Kroutil, W.; Pohl, M.; Rother, D.
    Biochemical characterization of an alcohol dehydrogenase from Ralstonia sp. (2013), Biotechnol. Bioeng., 110, 1838-1848.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.71 expressed in Escherichia coli BL21(DE3) cells Ralstonia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.71 (R)-2-hydroxypropiophenone
-
 Ralstonia sp.
1.1.1.71 Cyclohexanol
-
 Ralstonia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.71 0.005
-
 NADPH with benzaldehyde as cosubstrate, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 0.008
-
 NADPH with cyclohexanone as cosubstrate, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 0.06
-
 NADP+ with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C Ralstonia sp.
1.1.1.71 1.6
-
 (R)-2-hydroxypropiophenone at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 3.6
-
 (S)-2-Hydroxypropiophenone at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 5.2
-
 Cyclohexanol at pH 9.0 and 30°C Ralstonia sp.
1.1.1.71 10.8
-
 cyclohexanone at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 12.3
-
 benzyl alcohol at pH 9.0 and 30°C Ralstonia sp.
1.1.1.71 15.6
-
 benzaldehyde at pH 7.5 and 30°C Ralstonia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.71 Ca2+ Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained Ralstonia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Ralstonia sp.
-
-
-
1.1.1.71 Ralstonia sp. DSM 6428
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71
-
 Ralstonia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.71 0.01
-
 with acetone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 0.05
-
 with acetaldehyde, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 0.08
-
 with 2,5-hexandione, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 0.11
-
 with cyclohexanone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 0.56
-
 with 4-phenyl-2-butanone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 4.35
-
 with acetophenone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 4.89
-
 with benzaldehyde, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 8.36
-
 with cyclohexanol, at pH 10.5 and 30°C Ralstonia sp.
1.1.1.71 9.78
-
 with cyclohexanone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 11.58
-
 with 3-methylcyclohexanol, at pH 10.5 and 30°C Ralstonia sp.
1.1.1.71 11.7
-
 with 3-fluorobenzaldehyde, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 12.62
-
 with 3-methylcyclohexanone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 17.1
-
 with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 18.83
-
 with cyclohexenol, at pH 10.5 and 30°C Ralstonia sp.
1.1.1.71 23.31
-
 2-bromobenzaldehyde, at pH 7.5 and 30°C Ralstonia sp.
1.1.1.71 362.6
-
 with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C Ralstonia sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.71 -20°C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity Ralstonia sp.
1.1.1.71 8°C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity Ralstonia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.71 (1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+
-
 Ralstonia sp. (1R)-1-phenylpropane-1,2-diol + NADP+
-
 ?
1.1.1.71 (2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
 Ralstonia sp. (2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
 ?
1.1.1.71 (2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
 Ralstonia sp. (2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
 ?
1.1.1.71 (R)-2-hydroxypropiophenone + NADPH + H+
-
 Ralstonia sp. ?
-
 r
1.1.1.71 (S)-2-hydroxypropiophenone + NADPH + H+
-
 Ralstonia sp. ?
-
 r
1.1.1.71 2,5-hexandione + NADH + H+
-
 Ralstonia sp. ?
-
 r
1.1.1.71 2-bromobenzaldehyde + NADPH
-
 Ralstonia sp. 2-bromobenzyl alcohol + NADP+
-
 r
1.1.1.71 2-bromobenzaldehyde + NADPH + H+
-
 Ralstonia sp. 2-bromobenzyl alcohol + NADP+
-
 ?
1.1.1.71 2-cyclohexenol + NADP+
-
 Ralstonia sp. 2-cyclohexenone + NADH + H+
-
 r
1.1.1.71 2-cyclohexenone + NADH + H+
-
 Ralstonia sp. 2-cyclohexenol + NADP+
-
 r
1.1.1.71 2-fluorobenzaldehyde + NADPH + H+
-
 Ralstonia sp. 2-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.71 2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+
-
 Ralstonia sp. 2-methyl-1-phenylpropan-1,2-diol + NADP+
-
 ?
1.1.1.71 2-methoxybenzaldehyde + NADPH + H+
-
 Ralstonia sp. 2-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.71 2-methoxybenzaldehyde + NADPH + H+
-
 Ralstonia sp. DSM 6428 2-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.71 2-methylbenzaldehyde + NADPH + H+
-
 Ralstonia sp. 2-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.71 3-fluorobenzaldehyde + NADPH
-
 Ralstonia sp. 3-fluorobenzyl alcohol + NADP+
-
 r
1.1.1.71 3-methoxybenzaldehyde + NADPH + H+
-
 Ralstonia sp. 3-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.71 3-methylbenzaldehyde + NADPH + H+
-
 Ralstonia sp. 3-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.71 3-methylcyclohexanol + NADP+
-
 Ralstonia sp. ?
-
 r
1.1.1.71 3-methylcyclohexanone + NADH + H+
-
 Ralstonia sp. 3-methylcyclohexanol + NAD+
-
 r
1.1.1.71 4-bromobenzaldehyde + NADPH + H+
-
 Ralstonia sp. 4-bromobenzyl alcohol + NADP+
-
 ?
1.1.1.71 4-fluorobenzaldehyde + NADPH + H+
-
 Ralstonia sp. 4-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.71 4-methoxybenzaldehyde + NADPH + H+
-
 Ralstonia sp. 4-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.71 4-methoxybenzaldehyde + NADPH + H+
-
 Ralstonia sp. DSM 6428 4-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.71 4-methylbenzaldehyde + NADPH + H+
-
 Ralstonia sp. 4-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.71 4-methylpentan-2-one + NADPH + H+
-
 Ralstonia sp. 4-methylpentan-2-ol + NADP+
-
 ?
1.1.1.71 4-phenyl-2-butanone + NADH + H+
-
 Ralstonia sp. 4-phenyl-2-butanol + NAD+
-
 r
1.1.1.71 acetaldehyde + NADPH
-
 Ralstonia sp. ethanol + NADP+
-
 r
1.1.1.71 acetone + NADH + H+
-
 Ralstonia sp. propan-2-ol + NAD+
-
 r
1.1.1.71 acetophenone + NADH + H+
-
 Ralstonia sp. 1-phenylethan-1-ol + NAD+
-
 r
1.1.1.71 benzaldehyde + NADPH
-
 Ralstonia sp. benzyl alcohol + NADP+
-
 r
1.1.1.71 benzaldehyde + NADPH
-
 Ralstonia sp. DSM 6428 benzyl alcohol + NADP+
-
 r
1.1.1.71 benzyl alcohol + NADP+
-
 Ralstonia sp. benzaldehyde + NADPH
-
 r
1.1.1.71 benzyl alcohol + NADP+
-
 Ralstonia sp. DSM 6428 benzaldehyde + NADPH
-
 r
1.1.1.71 butan-2-ol + NADP+
-
 Ralstonia sp. butan-2-one + NADPH + H+
-
 ?
1.1.1.71 butan-2-one + NADPH + H+
-
 Ralstonia sp. butan-2-ol + NADP+
-
 ?
1.1.1.71 cyclohexanol + NADP+
-
 Ralstonia sp. cyclohexanone + NADH + H+
-
 r
1.1.1.71 cyclohexanone + NADH + H+
-
 Ralstonia sp. cyclohexanol + NAD+
-
 r
1.1.1.71 cyclohexanone + NADH + H+
-
 Ralstonia sp. cyclohexanol + NADP+
-
 r
1.1.1.71 formaldehyde + NADPH + H+
-
 Ralstonia sp. methanol + NADP+
-
 ?
1.1.1.71 furan-2-carbaldehyde + NADPH + H+
-
 Ralstonia sp. (furan-2-yl)methanol + NADP+
-
 ?
1.1.1.71 heptan-4-one + NADPH + H+
-
 Ralstonia sp. heptan-4-ol + NADP+
-
 ?
1.1.1.71 hexan-1-ol + NADP+
-
 Ralstonia sp. hexaldehyde + NADPH + H+
-
 ?
1.1.1.71 hexan-2-one + NADPH + H+
-
 Ralstonia sp. hexan-2-ol + NADP+
-
 ?
1.1.1.71 hexanal + NADPH + H+
-
 Ralstonia sp. hexan-1-ol + NADP+
-
 ?
1.1.1.71 additional information no activity with methanol and ethanol Ralstonia sp. ?
-
 ?
1.1.1.71 additional information no activity with methanol and ethanol Ralstonia sp. DSM 6428 ?
-
 ?
1.1.1.71 octan-1-ol + NADP+
-
 Ralstonia sp. octaldehyde + NADPH + H+
-
 ?
1.1.1.71 octan-2-one + NADPH + H+
-
 Ralstonia sp. octan-2-ol + NADP+
-
 ?
1.1.1.71 pentan-1-ol + NADP+
-
 Ralstonia sp. pentaldehyde + NADPH + H+
-
 ?
1.1.1.71 propan-1-ol + NADP+
-
 Ralstonia sp. propionaldehyde + NADPH + H+
-
 ?
1.1.1.71 propan-2-ol + NADP+
-
 Ralstonia sp. propan-2-one + NADPH + H+
-
 ?
1.1.1.71 pyridine-2-carbaldehyde + NADPH + H+
-
 Ralstonia sp. (pyridin-2-yl)methanol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.71 NADPH-dependent alcohol dehydrogenase
-
 Ralstonia sp.
1.1.1.71 RADH
-
 Ralstonia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.71 8 25 the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h) Ralstonia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.71 6 9.5 reduction reaction Ralstonia sp.
1.1.1.71 10 11.5 oxidation reaction Ralstonia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.71 5.5 8 the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature Ralstonia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.71 NADP+
-
 Ralstonia sp.
1.1.1.71 NADPH
-
 Ralstonia sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.71 11.4
-
 Cyclohexanol at pH 9.0 and 30°C Ralstonia sp.
1.1.1.71 16.4
-
 (R)-2-hydroxypropiophenone at pH 7.5 and 30°C Ralstonia sp.