BRENDA - Enzyme Database

Biochemical characterization of an alcohol dehydrogenase from Ralstonia sp.

Kulig, J.; Frese, A.; Kroutil, W.; Pohl, M.; Rother, D.; Biotechnol. Bioeng. 110, 1838-1848 (2013) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.71
expressed in Escherichia coli BL21(DE3) cells
Ralstonia sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.71
(R)-2-hydroxypropiophenone
-
Ralstonia sp.
1.1.1.71
Cyclohexanol
-
Ralstonia sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.71
0.005
-
NADPH
with benzaldehyde as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.008
-
NADPH
with cyclohexanone as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.06
-
NADP+
with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
1.6
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
3.6
-
(S)-2-Hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
5.2
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
10.8
-
cyclohexanone
at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
12.3
-
benzyl alcohol
at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
15.6
-
benzaldehyde
at pH 7.5 and 30°C
Ralstonia sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.71
Ca2+
Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained
Ralstonia sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.71
Ralstonia sp.
-
-
-
1.1.1.71
Ralstonia sp. DSM 6428
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.71
-
Ralstonia sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.71
0.01
-
with acetone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.05
-
with acetaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.08
-
with 2,5-hexandione, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.11
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.56
-
with 4-phenyl-2-butanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
4.35
-
with acetophenone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
4.89
-
with benzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
8.36
-
with cyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
1.1.1.71
9.78
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
11.58
-
with 3-methylcyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
1.1.1.71
11.7
-
with 3-fluorobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
12.62
-
with 3-methylcyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
17.1
-
with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
18.83
-
with cyclohexenol, at pH 10.5 and 30°C
Ralstonia sp.
1.1.1.71
23.31
-
2-bromobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
362.6
-
with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.71
-20°C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity
Ralstonia sp.
1.1.1.71
8°C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity
Ralstonia sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.71
(1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+
-
740200
Ralstonia sp.
(1R)-1-phenylpropane-1,2-diol + NADP+
-
-
-
?
1.1.1.71
(2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
1.1.1.71
(2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
1.1.1.71
(R)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
(S)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
2,5-hexandione + NADH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
2-bromobenzaldehyde + NADPH
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
r
1.1.1.71
2-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-cyclohexenol + NADP+
-
740200
Ralstonia sp.
2-cyclohexenone + NADH + H+
-
-
-
r
1.1.1.71
2-cyclohexenone + NADH + H+
-
740200
Ralstonia sp.
2-cyclohexenol + NADP+
-
-
-
r
1.1.1.71
2-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-fluorobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+
-
740200
Ralstonia sp.
2-methyl-1-phenylpropan-1,2-diol + NADP+
-
-
-
?
1.1.1.71
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
2-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methylbenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
3-fluorobenzaldehyde + NADPH
-
740200
Ralstonia sp.
3-fluorobenzyl alcohol + NADP+
-
-
-
r
1.1.1.71
3-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
3-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methylbenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
3-methylcyclohexanol + NADP+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
3-methylcyclohexanone + NADH + H+
-
740200
Ralstonia sp.
3-methylcyclohexanol + NAD+
-
-
-
r
1.1.1.71
4-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-bromobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-fluorobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
4-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methylbenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methylpentan-2-one + NADPH + H+
-
740200
Ralstonia sp.
4-methylpentan-2-ol + NADP+
-
-
-
?
1.1.1.71
4-phenyl-2-butanone + NADH + H+
-
740200
Ralstonia sp.
4-phenyl-2-butanol + NAD+
-
-
-
r
1.1.1.71
acetaldehyde + NADPH
-
740200
Ralstonia sp.
ethanol + NADP+
-
-
-
r
1.1.1.71
acetone + NADH + H+
-
740200
Ralstonia sp.
propan-2-ol + NAD+
-
-
-
r
1.1.1.71
acetophenone + NADH + H+
-
740200
Ralstonia sp.
1-phenylethan-1-ol + NAD+
-
-
-
r
1.1.1.71
benzaldehyde + NADPH
-
740200
Ralstonia sp.
benzyl alcohol + NADP+
-
-
-
r
1.1.1.71
benzaldehyde + NADPH
-
740200
Ralstonia sp. DSM 6428
benzyl alcohol + NADP+
-
-
-
r
1.1.1.71
benzyl alcohol + NADP+
-
740200
Ralstonia sp.
benzaldehyde + NADPH
-
-
-
r
1.1.1.71
benzyl alcohol + NADP+
-
740200
Ralstonia sp. DSM 6428
benzaldehyde + NADPH
-
-
-
r
1.1.1.71
butan-2-ol + NADP+
-
740200
Ralstonia sp.
butan-2-one + NADPH + H+
-
-
-
?
1.1.1.71
butan-2-one + NADPH + H+
-
740200
Ralstonia sp.
butan-2-ol + NADP+
-
-
-
?
1.1.1.71
cyclohexanol + NADP+
-
740200
Ralstonia sp.
cyclohexanone + NADH + H+
-
-
-
r
1.1.1.71
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NAD+
-
-
-
r
1.1.1.71
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NADP+
-
-
-
r
1.1.1.71
formaldehyde + NADPH + H+
-
740200
Ralstonia sp.
methanol + NADP+
-
-
-
?
1.1.1.71
furan-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(furan-2-yl)methanol + NADP+
-
-
-
?
1.1.1.71
heptan-4-one + NADPH + H+
-
740200
Ralstonia sp.
heptan-4-ol + NADP+
-
-
-
?
1.1.1.71
hexan-1-ol + NADP+
-
740200
Ralstonia sp.
hexaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
hexan-2-one + NADPH + H+
-
740200
Ralstonia sp.
hexan-2-ol + NADP+
-
-
-
?
1.1.1.71
hexanal + NADPH + H+
-
740200
Ralstonia sp.
hexan-1-ol + NADP+
-
-
-
?
1.1.1.71
additional information
no activity with methanol and ethanol
740200
Ralstonia sp.
?
-
-
-
?
1.1.1.71
additional information
no activity with methanol and ethanol
740200
Ralstonia sp. DSM 6428
?
-
-
-
?
1.1.1.71
octan-1-ol + NADP+
-
740200
Ralstonia sp.
octaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
octan-2-one + NADPH + H+
-
740200
Ralstonia sp.
octan-2-ol + NADP+
-
-
-
?
1.1.1.71
pentan-1-ol + NADP+
-
740200
Ralstonia sp.
pentaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
propan-1-ol + NADP+
-
740200
Ralstonia sp.
propionaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
propan-2-ol + NADP+
-
740200
Ralstonia sp.
propan-2-one + NADPH + H+
-
-
-
?
1.1.1.71
pyridine-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(pyridin-2-yl)methanol + NADP+
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.1.71
NADPH-dependent alcohol dehydrogenase
-
Ralstonia sp.
1.1.1.71
RADH
-
Ralstonia sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.71
8
25
the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)
Ralstonia sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.71
6
9.5
reduction reaction
Ralstonia sp.
1.1.1.71
10
11.5
oxidation reaction
Ralstonia sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.71
5.5
8
the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature
Ralstonia sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.71
NADP+
-
Ralstonia sp.
1.1.1.71
NADPH
-
Ralstonia sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.71
11.4
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
16.4
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.71
expressed in Escherichia coli BL21(DE3) cells
Ralstonia sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.71
NADP+
-
Ralstonia sp.
1.1.1.71
NADPH
-
Ralstonia sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.71
(R)-2-hydroxypropiophenone
-
Ralstonia sp.
1.1.1.71
Cyclohexanol
-
Ralstonia sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.71
11.4
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
16.4
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.71
0.005
-
NADPH
with benzaldehyde as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.008
-
NADPH
with cyclohexanone as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.06
-
NADP+
with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
1.6
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
3.6
-
(S)-2-Hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
5.2
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
10.8
-
cyclohexanone
at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
12.3
-
benzyl alcohol
at pH 9.0 and 30°C
Ralstonia sp.
1.1.1.71
15.6
-
benzaldehyde
at pH 7.5 and 30°C
Ralstonia sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.71
Ca2+
Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained
Ralstonia sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.71
-
Ralstonia sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.71
0.01
-
with acetone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.05
-
with acetaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.08
-
with 2,5-hexandione, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.11
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
0.56
-
with 4-phenyl-2-butanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
4.35
-
with acetophenone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
4.89
-
with benzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
8.36
-
with cyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
1.1.1.71
9.78
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
11.58
-
with 3-methylcyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
1.1.1.71
11.7
-
with 3-fluorobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
12.62
-
with 3-methylcyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
17.1
-
with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
18.83
-
with cyclohexenol, at pH 10.5 and 30°C
Ralstonia sp.
1.1.1.71
23.31
-
2-bromobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
1.1.1.71
362.6
-
with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.71
-20°C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity
Ralstonia sp.
1.1.1.71
8°C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity
Ralstonia sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.71
(1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+
-
740200
Ralstonia sp.
(1R)-1-phenylpropane-1,2-diol + NADP+
-
-
-
?
1.1.1.71
(2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
1.1.1.71
(2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
1.1.1.71
(R)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
(S)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
2,5-hexandione + NADH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
2-bromobenzaldehyde + NADPH
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
r
1.1.1.71
2-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-cyclohexenol + NADP+
-
740200
Ralstonia sp.
2-cyclohexenone + NADH + H+
-
-
-
r
1.1.1.71
2-cyclohexenone + NADH + H+
-
740200
Ralstonia sp.
2-cyclohexenol + NADP+
-
-
-
r
1.1.1.71
2-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-fluorobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+
-
740200
Ralstonia sp.
2-methyl-1-phenylpropan-1,2-diol + NADP+
-
-
-
?
1.1.1.71
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
2-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
2-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methylbenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
3-fluorobenzaldehyde + NADPH
-
740200
Ralstonia sp.
3-fluorobenzyl alcohol + NADP+
-
-
-
r
1.1.1.71
3-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
3-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methylbenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
3-methylcyclohexanol + NADP+
-
740200
Ralstonia sp.
?
-
-
-
r
1.1.1.71
3-methylcyclohexanone + NADH + H+
-
740200
Ralstonia sp.
3-methylcyclohexanol + NAD+
-
-
-
r
1.1.1.71
4-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-bromobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-fluorobenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
4-methoxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methylbenzyl alcohol + NADP+
-
-
-
?
1.1.1.71
4-methylpentan-2-one + NADPH + H+
-
740200
Ralstonia sp.
4-methylpentan-2-ol + NADP+
-
-
-
?
1.1.1.71
4-phenyl-2-butanone + NADH + H+
-
740200
Ralstonia sp.
4-phenyl-2-butanol + NAD+
-
-
-
r
1.1.1.71
acetaldehyde + NADPH
-
740200
Ralstonia sp.
ethanol + NADP+
-
-
-
r
1.1.1.71
acetone + NADH + H+
-
740200
Ralstonia sp.
propan-2-ol + NAD+
-
-
-
r
1.1.1.71
acetophenone + NADH + H+
-
740200
Ralstonia sp.
1-phenylethan-1-ol + NAD+
-
-
-
r
1.1.1.71
benzaldehyde + NADPH
-
740200
Ralstonia sp.
benzyl alcohol + NADP+
-
-
-
r
1.1.1.71
benzaldehyde + NADPH
-
740200
Ralstonia sp. DSM 6428
benzyl alcohol + NADP+
-
-
-
r
1.1.1.71
benzyl alcohol + NADP+
-
740200
Ralstonia sp.
benzaldehyde + NADPH
-
-
-
r
1.1.1.71
benzyl alcohol + NADP+
-
740200
Ralstonia sp. DSM 6428
benzaldehyde + NADPH
-
-
-
r
1.1.1.71
butan-2-ol + NADP+
-
740200
Ralstonia sp.
butan-2-one + NADPH + H+
-
-
-
?
1.1.1.71
butan-2-one + NADPH + H+
-
740200
Ralstonia sp.
butan-2-ol + NADP+
-
-
-
?
1.1.1.71
cyclohexanol + NADP+
-
740200
Ralstonia sp.
cyclohexanone + NADH + H+
-
-
-
r
1.1.1.71
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NAD+
-
-
-
r
1.1.1.71
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NADP+
-
-
-
r
1.1.1.71
formaldehyde + NADPH + H+
-
740200
Ralstonia sp.
methanol + NADP+
-
-
-
?
1.1.1.71
furan-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(furan-2-yl)methanol + NADP+
-
-
-
?
1.1.1.71
heptan-4-one + NADPH + H+
-
740200
Ralstonia sp.
heptan-4-ol + NADP+
-
-
-
?
1.1.1.71
hexan-1-ol + NADP+
-
740200
Ralstonia sp.
hexaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
hexan-2-one + NADPH + H+
-
740200
Ralstonia sp.
hexan-2-ol + NADP+
-
-
-
?
1.1.1.71
hexanal + NADPH + H+
-
740200
Ralstonia sp.
hexan-1-ol + NADP+
-
-
-
?
1.1.1.71
additional information
no activity with methanol and ethanol
740200
Ralstonia sp.
?
-
-
-
?
1.1.1.71
additional information
no activity with methanol and ethanol
740200
Ralstonia sp. DSM 6428
?
-
-
-
?
1.1.1.71
octan-1-ol + NADP+
-
740200
Ralstonia sp.
octaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
octan-2-one + NADPH + H+
-
740200
Ralstonia sp.
octan-2-ol + NADP+
-
-
-
?
1.1.1.71
pentan-1-ol + NADP+
-
740200
Ralstonia sp.
pentaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
propan-1-ol + NADP+
-
740200
Ralstonia sp.
propionaldehyde + NADPH + H+
-
-
-
?
1.1.1.71
propan-2-ol + NADP+
-
740200
Ralstonia sp.
propan-2-one + NADPH + H+
-
-
-
?
1.1.1.71
pyridine-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(pyridin-2-yl)methanol + NADP+
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.71
8
25
the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)
Ralstonia sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.71
6
9.5
reduction reaction
Ralstonia sp.
1.1.1.71
10
11.5
oxidation reaction
Ralstonia sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.71
5.5
8
the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature
Ralstonia sp.