BRENDA - Enzyme Database

Characterization of NADP(+)-dependent isocitrate dehydrogenase isozymes from a psychrophilic bacterium, Colwellia psychrerythraea strain 34H

Suzuki, K.; Takada, Y.; Biosci. Biotechnol. Biochem. 80, 1492-1498 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
gene icd-D or icd-I, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-I in IDH-deficient Escherichia coli strain DEK2004; gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIa in IDH-deficient Escherichia coli strain DEK2004; gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIb in IDH-deficient Escherichia coli strain DEK2004
Colwellia psychrerythraea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
activates; activates
Colwellia psychrerythraea
1.1.1.42
Mn2+
activates; activates
Colwellia psychrerythraea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Colwellia psychrerythraea
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
1.1.1.42
isocitrate + NADP+
Colwellia psychrerythraea DSM 8813
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Colwellia psychrerythraea
Q1XIQ8
-
-
1.1.1.42
Colwellia psychrerythraea
Q1XIQ9
-
-
1.1.1.42
Colwellia psychrerythraea DSM 8813
Q1XIQ8
-
-
1.1.1.42
Colwellia psychrerythraea DSM 8813
Q1XIQ9
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
additional information
no cell growth at 15-37°C; no cell growth at 15-37°C
Colwellia psychrerythraea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
740185
Colwellia psychrerythraea
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
740185
Colwellia psychrerythraea DSM 8813
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
homodimer
-
Colwellia psychrerythraea
1.1.1.42
monomer
-
Colwellia psychrerythraea
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
20
-
assay at
Colwellia psychrerythraea
1.1.1.42
30
-
assay at
Colwellia psychrerythraea
1.1.1.42
50
-
optimum of the IDH overall activity in Colwellia psychrerythraea crude cell extract; optimum of the IDH overall activity in Colwellia psychrerythraea crude cell extract
Colwellia psychrerythraea
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.42
additional information
-
isozyme IDH-IIa is a cold-adapted enzyme; isozyme IDH-IIa is a mesophilic enzyme; isozyme IDH-IIb is a cold-adapted enzyme
Colwellia psychrerythraea
1.1.1.42
10
60
activity range of the IDH overall activity in Colwellia psychrerythraea crude cell extract, inactivation above; activity range of the IDH overall activity in Colwellia psychrerythraea crude cell extract, inactivation above
Colwellia psychrerythraea
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
10
15
stable at, purified recombinant His-tagged isozyme IDH-IIa; stable at, purified recombinant His-tagged isozyme IDH-IIb
Colwellia psychrerythraea
1.1.1.42
40
-
inactivation, purified recombinant His-tagged isozyme IDH-IIa; inactivation, purified recombinant His-tagged isozyme IDH-IIb; stable up to, purified recombinant His-tagged isozyme IDH-I
Colwellia psychrerythraea
1.1.1.42
50
-
inactivation, purified recombinant His-tagged isozyme IDH-I
Colwellia psychrerythraea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
assay at; assay at
Colwellia psychrerythraea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
;
Colwellia psychrerythraea
1.1.1.42
NADPH
;
Colwellia psychrerythraea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
gene icd-D or icd-I, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-I in IDH-deficient Escherichia coli strain DEK2004
Colwellia psychrerythraea
1.1.1.42
gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIa in IDH-deficient Escherichia coli strain DEK2004; gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIb in IDH-deficient Escherichia coli strain DEK2004
Colwellia psychrerythraea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Colwellia psychrerythraea
1.1.1.42
NADPH
-
Colwellia psychrerythraea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
activates
Colwellia psychrerythraea
1.1.1.42
Mn2+
activates
Colwellia psychrerythraea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Colwellia psychrerythraea
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
1.1.1.42
isocitrate + NADP+
Colwellia psychrerythraea DSM 8813
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
additional information
no cell growth at 15-37°C
Colwellia psychrerythraea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
740185
Colwellia psychrerythraea
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
740185
Colwellia psychrerythraea DSM 8813
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
homodimer
-
Colwellia psychrerythraea
1.1.1.42
monomer
-
Colwellia psychrerythraea
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
20
-
assay at
Colwellia psychrerythraea
1.1.1.42
30
-
assay at
Colwellia psychrerythraea
1.1.1.42
50
-
optimum of the IDH overall activity in Colwellia psychrerythraea crude cell extract
Colwellia psychrerythraea
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.42
additional information
-
isozyme IDH-IIa is a mesophilic enzyme
Colwellia psychrerythraea
1.1.1.42
additional information
-
isozyme IDH-IIa is a cold-adapted enzyme; isozyme IDH-IIb is a cold-adapted enzyme
Colwellia psychrerythraea
1.1.1.42
10
60
activity range of the IDH overall activity in Colwellia psychrerythraea crude cell extract, inactivation above
Colwellia psychrerythraea
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
10
15
stable at, purified recombinant His-tagged isozyme IDH-IIa; stable at, purified recombinant His-tagged isozyme IDH-IIb
Colwellia psychrerythraea
1.1.1.42
40
-
stable up to, purified recombinant His-tagged isozyme IDH-I
Colwellia psychrerythraea
1.1.1.42
40
-
inactivation, purified recombinant His-tagged isozyme IDH-IIa; inactivation, purified recombinant His-tagged isozyme IDH-IIb
Colwellia psychrerythraea
1.1.1.42
50
-
inactivation, purified recombinant His-tagged isozyme IDH-I
Colwellia psychrerythraea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
assay at
Colwellia psychrerythraea
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
evolution
three NADP+-dependent isocitrate dehydrogenase (IDH) isozymes of a psychrophilic bacterium, Colwellia psychrerythraea strain 34H, are analyzed: two monomeric (IDH-IIa and IDH-IIb) and one dimeric (IDH-I) IDHs; three NADP+-dependent isocitrate dehydrogenase (IDH) isozymes of a psychrophilic bacterium, Colwellia psychrerythraea strain 34H, are analyzed: two monomeric (IDH-IIa and IDH-IIb) and one dimeric (IDH-I) IDHs
Colwellia psychrerythraea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
evolution
three NADP+-dependent isocitrate dehydrogenase (IDH) isozymes of a psychrophilic bacterium, Colwellia psychrerythraea strain 34H, are analyzed: two monomeric (IDH-IIa and IDH-IIb) and one dimeric (IDH-I) IDHs
Colwellia psychrerythraea