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Literature summary extracted from

  • Araki, T.; Nakatsuka, T.; Kobayashi, F.; Watanabe-Ishimaru, E.; Sanada, H.; Tamura, T.; Inagaki, K.
    Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol (2015), Biosci. Biotechnol. Biochem., 79, 1130-1132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.32 expressed in Escherichia coli BL21(DE3) cells Sinorhizobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.32 62.4
-
 L-sorbose at pH 8.5 and 40°C Sinorhizobium sp.
1.1.99.32 97.5
-
 1,5-anhydro-D-glucitol at pH 8.5 and 40°C Sinorhizobium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.32 membrane
-
 Sinorhizobium sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.32 59000
-
 2 * 59000, SDS-PAGE Sinorhizobium sp.
1.1.99.32 150000
-
 gel filtration Sinorhizobium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.32 Sinorhizobium sp.
-
-
-
1.1.99.32 Sinorhizobium sp. 97507
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.32 TALON metal affinity resin column chromatography Sinorhizobium sp.

Storage Stability

EC Number Storage Stability Organism
1.1.99.32 4°C, purified enzyme, 2 days, 30% loss of activity Sinorhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.32 1,5-anhydro-D-glucitol + 2,6-dichlorophenolindophenol 79% activity compared to L-sorbose Sinorhizobium sp. ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 1,5-anhydro-D-glucitol + 2,6-dichlorophenolindophenol 79% activity compared to L-sorbose Sinorhizobium sp. 97507 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 D-galactose + 2,6-dichlorophenolindophenol 1.4% activity compared to L-sorbose Sinorhizobium sp. ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 D-galactose + 2,6-dichlorophenolindophenol 1.4% activity compared to L-sorbose Sinorhizobium sp. 97507 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 D-glucose + 2,6-dichlorophenolindophenol 1.0% activity compared to L-sorbose Sinorhizobium sp. ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 D-glucose + 2,6-dichlorophenolindophenol 1.0% activity compared to L-sorbose Sinorhizobium sp. 97507 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 L-sorbose + 2,6-dichlorophenolindophenol 100% activity Sinorhizobium sp. L-sorbosone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 L-sorbose + 2,6-dichlorophenolindophenol 100% activity Sinorhizobium sp. 97507 L-sorbosone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.32 additional information less than 1% activity with D-fructose, D-cellobiose, 2-deoxy-D-glucose, methyl-beta-D-glucopyranoside, 3-O-methyl-D-glucose, D-glucose 6 phosphate, D-mannose, D-sucrose, D-arabinose, and D-raffinose. No activity with arabitol, D-xylose, and glycerol Sinorhizobium sp. ?
-
 ?
1.1.99.32 additional information less than 1% activity with D-fructose, D-cellobiose, 2-deoxy-D-glucose, methyl-beta-D-glucopyranoside, 3-O-methyl-D-glucose, D-glucose 6 phosphate, D-mannose, D-sucrose, D-arabinose, and D-raffinose. No activity with arabitol, D-xylose, and glycerol Sinorhizobium sp. 97507 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.32 homodimer 2 * 59000, SDS-PAGE Sinorhizobium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.99.32 SDH
-
 Sinorhizobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.32 40
-
-
 Sinorhizobium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.32 30 55 the enzyme is stable at temperatures of up to 30°C, and 82% of enzyme activity persists at 35°C. Enzyme activity is almost completely abrogated by a 10 min incubation at 55°C Sinorhizobium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.32 11.2
-
 L-sorbose at pH 8.5 and 40°C Sinorhizobium sp.
1.1.99.32 14.1
-
 1,5-anhydro-D-glucitol at pH 8.5 and 40°C Sinorhizobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.32 8.5
-
-
 Sinorhizobium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.32 5.5 8 no decrease in activity is observed in the pH range 5.5-8.0 Sinorhizobium sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.32 0.15
-
 1,5-anhydro-D-glucitol at pH 8.5 and 40°C Sinorhizobium sp.
1.1.99.32 0.18
-
 L-sorbose at pH 8.5 and 40°C Sinorhizobium sp.