BRENDA - Enzyme Database

Overexpression of the NADP+-specific isocitrate dehydrogenase gene (icdA) in citric acid-producing Aspergillus niger WU-2223L

Kobayashi, K.; Hattori, T.; Hayashi, R.; Kirimura, K.; Biosci. Biotechnol. Biochem. 78, 1246-1253 (2014)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.1.1.42
biotechnology
the icdA gene is a potentially valuable tool for modulating citric acid production by metabolic engineering
Aspergillus niger
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
icdA, recombinant overexpression in Aspergillus niger strain WU-2223L
Aspergillus niger
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
construction of an NADP+-ICDH gene (icdA)-overexpressing strain (OPI-1) using Aspergillus niger strain WU-2223L. The amount of citric acid produced by Aspergillus niger can be altered with the NADP+-ICDH activity. Time-dependent transcriptional changes in the genes encoding NADP+-ICDH, NAD+-ICDH, and ICL are analyzed by RT-PCR. The glyoxylate cycle is poorly activated or slightly repressed in the icdA-overexpressing Aspergillus niger clone
Aspergillus niger
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Aspergillus niger
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Aspergillus niger
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
mycelium
-
Aspergillus niger
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
740181
Aspergillus niger
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Aspergillus niger
1.1.1.42
NADPH
-
Aspergillus niger
Application (protein specific)
EC Number
Application
Commentary
Organism
1.1.1.42
biotechnology
the icdA gene is a potentially valuable tool for modulating citric acid production by metabolic engineering
Aspergillus niger
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
icdA, recombinant overexpression in Aspergillus niger strain WU-2223L
Aspergillus niger
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Aspergillus niger
1.1.1.42
NADPH
-
Aspergillus niger
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
construction of an NADP+-ICDH gene (icdA)-overexpressing strain (OPI-1) using Aspergillus niger strain WU-2223L. The amount of citric acid produced by Aspergillus niger can be altered with the NADP+-ICDH activity. Time-dependent transcriptional changes in the genes encoding NADP+-ICDH, NAD+-ICDH, and ICL are analyzed by RT-PCR. The glyoxylate cycle is poorly activated or slightly repressed in the icdA-overexpressing Aspergillus niger clone
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Aspergillus niger
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
mycelium
-
Aspergillus niger
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
740181
Aspergillus niger
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
metabolism
in the tricarboxylic acid cycle, NADP+-specific isocitrate dehydrogenase catalyzes oxidative decarboxylation of isocitric acid to form 2-oxoglutaric acid with NADP+ as a cofactor. NADP+-ICDH may work independently of NAD+-ICDH, EC 1.1.1.41, as one of the TCA cycle regulatory enzymes, especially in relation to citric acid production by Aspergillus niger
Aspergillus niger
1.1.1.42
physiological function
ICDH is a key enzyme that regulates the TCA cycle
Aspergillus niger
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
metabolism
in the tricarboxylic acid cycle, NADP+-specific isocitrate dehydrogenase catalyzes oxidative decarboxylation of isocitric acid to form 2-oxoglutaric acid with NADP+ as a cofactor. NADP+-ICDH may work independently of NAD+-ICDH, EC 1.1.1.41, as one of the TCA cycle regulatory enzymes, especially in relation to citric acid production by Aspergillus niger
Aspergillus niger
1.1.1.42
physiological function
ICDH is a key enzyme that regulates the TCA cycle
Aspergillus niger