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Literature summary extracted from

  • Zhao, J.; Shi, P.; Li, Z.; Yang, P.; Luo, H., Bai, Y., Wang, Y.; Yao, B.
    Two neutral thermostable cellulases from Phialophora sp. G5 act synergistically in the hydrolysis of filter paper (2012), Bioresour. Technol., 121, 404-410.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 2-mercaptoethanol 118.4% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 2-mercaptoethanol 129.7% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 EDTA 99.4% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 EDTA 99.7% relative activity Phialophora sp. CGMCC 3328

Application

EC Number Application Comment Organism
3.2.1.4 industry textile and detergent industry, combination of CBH6A and EgGH45 Phialophora sp. CGMCC 3328

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expressed in Pichia pastoris Phialophora sp. CGMCC 3328

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Pb2+ no remaining activity; no remaining activity Phialophora sp. CGMCC 3328
3.2.1.4 SDS 10 mM, 35% remaining activity; 10 mM, 54% remaining activity Phialophora sp. CGMCC 3328

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethyl cellulose 46.2 mg/l and vmax: 17.1 micromol/min/mg, pH 7, 60°C Phialophora sp. CGMCC 3328
3.2.1.4 additional information
-
 carboxymethyl cellulose 71.9 mg/l and vmax: 28.6 micromol/min/mg, pH 7, 65°C Phialophora sp. CGMCC 3328

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 57.8% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Ca2+ 85.5% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Co2+ 69.2% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Co2+ 85.5% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Cr3+ 76.7% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Cr3+ 84.1% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Cu2+ 116.1% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Cu2+ 118% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Fe3+ 114.3% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Fe3+ 87.5% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 K+ 86.2% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 K+ 96.1% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Li+ 84.6% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Li+ 97% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Mg2+ 108.5% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Mg2+ 99.8% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Na+ 97.6% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Na+ 99.4% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Ni2+ 100.8% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Ni2+ 107.1% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Zn2+ 110% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 Zn2+ 99.9% relative activity Phialophora sp. CGMCC 3328

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 36000
-
 difference to 56000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328
3.2.1.4 36000
-
 difference to 56000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328
3.2.1.4 56000
-
 difference to 36000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328
3.2.1.4 56000
-
 difference to 36000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Phialophora sp. CGMCC 3328 F2VRY9 HM003039; G5 CGMCC 3328
-
3.2.1.4 Phialophora sp. CGMCC 3328 F2VRZ0 HM003040; G5 CGMCC 3328
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 centrifuged at 12,000 x g for 10 min to remove cell depris, hot shock, ultrafiltration, dialysis, gel filtration Phialophora sp. CGMCC 3328

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O 14% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 avicel + H2O 17.7% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 barley glucan + H2O 120% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 barley glucan + H2O 143% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 filter paper + H2O 4% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 filter paper + H2O 8% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 Phialophora sp. CGMCC 3328 ?
-
 ?
3.2.1.4 additional information no activity against birchwood xylan, 4-nitrophenyl cellobiose, 4-nitrophenyl beta-D-glucoside Phialophora sp. CGMCC 3328 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 cbh6A gene name, consists of a family 1 CBM and a catalytic domain of glycosyl hydrolase family 6 cellobiohydrolases Phialophora sp. CGMCC 3328
3.2.1.4 egGH45 gene name, contains a catalytic domain of family 45 endoglucanases Phialophora sp. CGMCC 3328

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
 50-70, determnined at 30-80°C, pH 6 for 30 min Phialophora sp. CGMCC 3328
3.2.1.4 65
-
 50-70, determnined at 30-80°C, pH 7 for 30 min Phialophora sp. CGMCC 3328

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 30 70 30°C: 20% relative activity, respectively, 70°C: 70% relative activity Phialophora sp. CGMCC 3328
3.2.1.4 30 70 30°C: 30% relative activity, respectively, 70°C: 70% relative activity Phialophora sp. CGMCC 3328

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
 CBH6A and EgGH45, 90% activity for 10 min Phialophora sp. CGMCC 3328
3.2.1.4 80
-
 CBH6A and EgGH45, 80% activity for 10 min Phialophora sp. CGMCC 3328
3.2.1.4 90
-
 CBH6A and EgGH45, 47% activity for 10 min Phialophora sp. CGMCC 3328

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6 8 EgGH45, measured in various buffers at pH 3-11 (McIlvaine, Tris-HCl, glycine-NaOH) at 60°C for 30 min Phialophora sp. CGMCC 3328
3.2.1.4 7
-
 CBH6A, measured in various buffers at pH 3-11 (McIlvaine, Tris-HCl, glycine-NaOH) at 60°C for 30 min Phialophora sp. CGMCC 3328

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 11 pH 3: 20% relative activity, pH 11: 35% relatice activity at 65°C Phialophora sp. CGMCC 3328
3.2.1.4 3 11 pH 3: 25% relative activity, pH 11: 40% relatice activity at 65°C Phialophora sp. CGMCC 3328

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 2 11 80-100% acticity, enzymes are preincubated in the buffers without substrate at 37°C for 2 h, and the residual activities are measured at optimal conditions for 30 min, 60°C and pH 6.0 for EgGH45 Phialophora sp. CGMCC 3328
3.2.1.4 2 11 80-100% activity, enzymes are preincubated in the buffers without substrate at 37°C for 2 h, and the residual activities are measured at optimal conditions for 30 min, 65°C and pH 7 Phialophora sp. CGMCC 3328