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Literature summary extracted from

  • Xue, Y.; Zhang, Y.; Wang, W.; Wang, Y.; Liu, Z.; Zou, S.; Zheng, Y.; Shen, Y.
    Highly enantioselective oxidation of alpha-hydroxyacids bearing a substituent with an aryl group: Co-production of optically active alpha-hydroxyacids and alpha-ketoacids (2013), Biores. Technol., 132, 391-394.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.B9 3.6
-
 (2S)-(4-bromophenyl)(hydroxy)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 3.7
-
 (2S)-(4-chlorophenyl)(hydroxy)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 4.9
-
 (2S)-(3-chlorophenyl)(hydroxy)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 8.6
-
 (2S)-hydroxy(3,5-difluorophenyl)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 11.5
-
 (2S)-(2-fluorophenyl)(hydroxy)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 15.2
-
 (2S)-hydroxy(2,4-difluorophenyl)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 64.9
-
 (2S)-hydroxy(phenyl)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 65.6
-
 (2S)-hydroxy(4-methylphenyl)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 66.5
-
 (2S)-(4-fluorophenyl)(hydroxy)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 68.5
-
 (2S)-hydroxy-3-phenylpropanoic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 73.5
-
 (2S)-hydroxy(4-hydroxyphenyl)acetic acid pH 8.0, 30°C Sinorhizobium sp.
1.1.99.B9 81.2
-
 (2S)-(2-chlorophenyl)(hydroxy)acetic acid pH 8.0, 30°C Sinorhizobium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B9 Sinorhizobium sp.
-
-
-
1.1.99.B9 Sinorhizobium sp. ZJB1101
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B9 (2S)-(2-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. (2-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(2-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. ZJB1101 (2-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(2-fluorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. (2-fluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(2-fluorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. ZJB1101 (2-fluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(3-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. (3-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(3-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. ZJB1101 (3-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(4-bromophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. (4-bromophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(4-bromophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. ZJB1101 (4-bromophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(4-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. (4-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-(4-fluorophenyl)(hydroxy)acetic acid + acceptor
-
 Sinorhizobium sp. (4-fluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-hydroxy(2,4-difluorophenyl)acetic acid + acceptor
-
 Sinorhizobium sp. (2,4-difluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-hydroxy(3,5-difluorophenyl)acetic acid + acceptor
-
 Sinorhizobium sp. (3,5-difluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-hydroxy(4-hydroxyphenyl)acetic acid + acceptor
-
 Sinorhizobium sp. (4-hydroxyphenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-hydroxy(4-methylphenyl)acetic acid + acceptor
-
 Sinorhizobium sp. (4-methylphenyl)(oxo)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-hydroxy(phenyl)acetic acid + acceptor
-
 Sinorhizobium sp. oxo(phenyl)acetic acid + reduced acceptor
-
 ?
1.1.99.B9 (2S)-hydroxy-3-phenylpropanoic acid + acceptor
-
 Sinorhizobium sp. 2-oxo-3-phenylpropanoic acid + reduced acceptor
-
 ?
1.1.99.B9 additional information enzyme is a stereoselective 2-hydroxyacid dehydrogenase (2-HADH) that can catalyze asymmetric oxidation of mandelic acid and 2-chloromandelic acid into corresponding 2-ketoacids and (R)-isomers with high activity and enantioselectivity, while no consecutive oxidation of 2-ketoacids is observed Sinorhizobium sp. ?
-
 ?
1.1.99.B9 additional information enzyme is a stereoselective 2-hydroxyacid dehydrogenase (2-HADH) that can catalyze asymmetric oxidation of mandelic acid and 2-chloromandelic acid into corresponding 2-ketoacids and (R)-isomers with high activity and enantioselectivity, while no consecutive oxidation of 2-ketoacids is observed Sinorhizobium sp. ZJB1101 ?
-
 ?