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Literature summary extracted from

  • Anand, A.; Jayaramaiah, R.H.; Beedkar, S.D.; Singh, P.A.; Joshi, R.S.; Mulani, F.A.; Dholakia, B.B.; Punekar, S.A.; Gade, W.N.; Thulasiram, H.V.; Giri, A.P.
    Comparative functional characterization of eugenol synthase from four different Ocimum species: Implications on eugenol accumulation (2016), Biochim. Biophys. Acta, 1864, 1539-1547.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.318 homology modeling of structure Ocimum gratissimum
1.1.1.318 homology modeling of structure Ocimum tenuiflorum
1.1.1.318 homology modeling of structure Ocimum kilimandscharicum
1.1.1.318 homology modeling of structure Ocimum basilicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.318 0.41
-
 coniferyl acetate pH 6.5, 25°C Ocimum kilimandscharicum
1.1.1.318 0.5
-
 coniferyl acetate pH 6.5, 25°C Ocimum gratissimum
1.1.1.318 0.55
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum kilimandscharicum
1.1.1.318 0.7
-
 coniferyl acetate pH 6.5, 25°C Ocimum basilicum
1.1.1.318 1.17
-
 coniferyl acetate pH 6.5, 25°C Ocimum tenuiflorum
1.1.1.318 1.25
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum gratissimum
1.1.1.318 1.7
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum tenuiflorum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.318 Ocimum basilicum A0A1B2U6R8
-
-
1.1.1.318 Ocimum gratissimum A0A1B2U6S7
-
-
1.1.1.318 Ocimum kilimandscharicum A0A1B2U6T4
-
-
1.1.1.318 Ocimum tenuiflorum A0A1B2U6S6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.318 flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum gratissimum
-
1.1.1.318 flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum tenuiflorum
-
1.1.1.318 flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum kilimandscharicum
-
1.1.1.318 flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum basilicum
-
1.1.1.318 leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum gratissimum
-
1.1.1.318 leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum tenuiflorum
-
1.1.1.318 leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum kilimandscharicum
-
1.1.1.318 leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum basilicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.318 4-coumaryl acetate + NADPH + H+
-
 Ocimum gratissimum chavicol + acetate + NADP+
-
 ?
1.1.1.318 4-coumaryl acetate + NADPH + H+
-
 Ocimum tenuiflorum chavicol + acetate + NADP+
-
 ?
1.1.1.318 4-coumaryl acetate + NADPH + H+
-
 Ocimum kilimandscharicum chavicol + acetate + NADP+
-
 ?
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum gratissimum eugenol + acetate + NADP+
-
 ?
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum tenuiflorum eugenol + acetate + NADP+
-
 ?
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum kilimandscharicum eugenol + acetate + NADP+
-
 ?
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum basilicum eugenol + acetate + NADP+
-
 ?
1.1.1.318 additional information no substrate: 4-coumaryl acetate, cinnamyl acetate Ocimum basilicum ?
-
 ?
1.1.1.318 additional information no substrate: cinnamyl acetate Ocimum gratissimum ?
-
 ?
1.1.1.318 additional information no substrate: cinnamyl acetate Ocimum tenuiflorum ?
-
 ?
1.1.1.318 additional information no substrate: cinnamyl acetate Ocimum kilimandscharicum ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.318 0.003
-
 coniferyl acetate pH 6.5, 25°C Ocimum kilimandscharicum
1.1.1.318 0.003
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum gratissimum
1.1.1.318 0.004
-
 coniferyl acetate pH 6.5, 25°C Ocimum basilicum
1.1.1.318 0.011
-
 coniferyl acetate pH 6.5, 25°C Ocimum gratissimum
1.1.1.318 0.011
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum kilimandscharicum
1.1.1.318 0.013
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum tenuiflorum
1.1.1.318 0.028
-
 coniferyl acetate pH 6.5, 25°C Ocimum tenuiflorum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.318 0.0027
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum gratissimum
1.1.1.318 0.003
-
 coniferyl acetate pH 6.5, 25°C Ocimum tenuiflorum
1.1.1.318 0.006
-
 coniferyl acetate pH 6.5, 25°C Ocimum basilicum
1.1.1.318 0.007
-
 coniferyl acetate pH 6.5, 25°C Ocimum kilimandscharicum
1.1.1.318 0.019
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum kilimandscharicum
1.1.1.318 0.023
-
 coniferyl acetate pH 6.5, 25°C Ocimum gratissimum
1.1.1.318 0.024
-
 4-coumaryl acetate pH 6.5, 25°C Ocimum tenuiflorum