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Literature summary extracted from

  • Stiti, N.; Podgorska, K.; Bartels, D.
    Aldehyde dehydrogenase enzyme ALDH3H1 from Arabidopsis thaliana: Identification of amino acid residues critical for cofactor specificity (2014), Biochim. Biophys. Acta, 1844, 681-693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 E149D the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149D/I200G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149D/I200V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149N the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149N/I200G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149N/I200V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149Q/I200G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149Q/I200V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149T the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149T/I200G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149T/I200V the mutant shows a good catalysis with NADP+ compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 E149T/V178R/I200V the mutant uses NADP+ with almost 7fold higher catalytic efficiency compared to NAD+ Arabidopsis thaliana
1.2.1.3 I200G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 I200V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.003
-
 trans-2-nonenal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.008
-
 Nonanal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.0322
-
 trans-2-nonenal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.065
-
 Nonanal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.071
-
 hexanal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.072
-
 hexanal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.18
-
 trans-2-hexenal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.199
-
 trans-2-hexenal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.421
-
 NAD+ wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.441
-
 NAD+ mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.496
-
 NAD+ mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.564
-
 NAD+ mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.63
-
 NAD+ mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.886
-
 NAD+ mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.991
-
 NAD+ mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 0.992
-
 NAD+ mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.068
-
 NAD+ mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.092
-
 NAD+ mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.262
-
 NAD+ mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.622
-
 NAD+ mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.648
-
 NAD+ mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.649
-
 NAD+ mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.829
-
 NAD+ mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.856
-
 NAD+ mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2.115
-
 NAD+ mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2.524
-
 NAD+ mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 3.218
-
 NAD+ mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 3.418
-
 NAD+ mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 3.651
-
 NAD+ mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 4.059
-
 NAD+ mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 4.136
-
 NAD+ mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Arabidopsis thaliana Q70DU8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 nickel affinity column chromatography, and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 hexanal + NAD+ + H2O
-
 Arabidopsis thaliana hexanoate + NADH + H+
-
 ?
1.2.1.3 additional information no activity with NADP+ Arabidopsis thaliana ?
-
 ?
1.2.1.3 nonanal + NAD+ + H2O
-
 Arabidopsis thaliana nonanoate + NADH + H+
-
 ?
1.2.1.3 trans-2-hexenal + NAD+ + H2O
-
 Arabidopsis thaliana (E)-hex-2-enoate + NADH + H+
-
 ?
1.2.1.3 trans-2-nonenal + NAD+ + H2O
-
 Arabidopsis thaliana (E)-non-2-enoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH3H1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 1.1
-
 trans-2-hexenal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 1.8
-
 NAD+ mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2.2
-
 trans-2-hexenal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2.2
-
 NAD+ mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2.7
-
 trans-2-nonenal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 3.1
-
 NAD+ mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 3.8
-
 trans-2-nonenal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 4
-
 NAD+ mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 8.2
-
 hexanal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 10.2
-
 NAD+ mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 10.7
-
 NAD+ mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 11.2
-
 hexanal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 11.4
-
 NAD+ mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 12.2
-
 NAD+ mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 12.4
-
 NAD+ mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 12.7
-
 NAD+ mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 12.7
-
 NAD+ mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 13
-
 Nonanal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 13.1
-
 NAD+ mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 15.7
-
 NAD+ mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 16.3
-
 NAD+ mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 16.6
-
 NAD+ mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 17.2
-
 NAD+ wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 17.9
-
 Nonanal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 18
-
 NAD+ mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 18.5
-
 NAD+ mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 20.1
-
 NAD+ mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 23.2
-
 NAD+ mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 26.9
-
 NAD+ mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 33.9
-
 NAD+ mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.3 1.1
-
 NAD+ mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2.8
-
 NAD+ mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 3.8
-
 NAD+ mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 4
-
 NAD+ mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 4.8
-
 NAD+ mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 5
-
 NAD+ mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 5.1
-
 NAD+ mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 5.3
-
 trans-2-hexenal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 6
-
 NAD+ mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 6.1
-
 NAD+ mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 6.4
-
 NAD+ mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 6.5
-
 NAD+ mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 8.1
-
 NAD+ mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 9.5
-
 NAD+ mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 9.9
-
 NAD+ mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 9.9
-
 NAD+ mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 12.4
-
 trans-2-hexenal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 12.5
-
 NAD+ mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 13.2
-
 NAD+ mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 14.3
-
 NAD+ mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 24.3
-
 NAD+ mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 26.3
-
 NAD+ mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 37.3
-
 NAD+ mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 40.9
-
 NAD+ wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 115
-
 hexanal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 120
-
 trans-2-nonenal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 154
-
 hexanal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 213
-
 Nonanal mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 918
-
 trans-2-nonenal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana
1.2.1.3 2166
-
 Nonanal wild type enzyme, at pH 8.0 and 25°C Arabidopsis thaliana