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Literature summary extracted from

  • Ouedraogo, D.; Souffrant, M.; Vasquez, S.; Hamelberg, D.; Gadda, G.
    Importance of loop L1 dynamics for substrate capture and catalysis in Pseudomonas aeruginosa D-arginine dehydrogenase (2017), Biochemistry, 56, 2477-2487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.99.6
-
 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.4.99.6 A46G mutation in loop L1. Probability of being in open conformation is higher than that of wild-type, yielding an increased level of solvent exposure of the active site. The flavin fluorescence intensity is about 2fold higher than in wild-type. Loop L1 is important for substrate capture and catalysis Pseudomonas aeruginosa
1.4.99.6 S45A mutation in loop L1. Probability of being in open conformation is higher than that of wild-type, yielding an increased level of solvent exposure of the active site. The flavin fluorescence intensity is about 2fold higher than in wild-type. Loop L1 is important for substrate capture and catalysis Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.6 Pseudomonas aeruginosa Q9HXE3
-
-
1.4.99.6 Pseudomonas aeruginosa DSM 22644 Q9HXE3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.6 D-arginine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate overall reaction ?
1.4.99.6 D-arginine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa DSM 22644 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate overall reaction ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.99.6 1.4
-
 D-arginine mutant S45A, pH 5.5, 25°C Pseudomonas aeruginosa
1.4.99.6 23
-
 D-arginine mutant A46G, pH 6.5, 25°C Pseudomonas aeruginosa
1.4.99.6 210
-
 D-arginine mutant A46G, pH 10.0, 25°C Pseudomonas aeruginosa
1.4.99.6 290
-
 D-arginine mutant S45A, pH 10.0, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.99.6 0.014
-
 D-arginine mutant S45A, pH 5.5, 25°C Pseudomonas aeruginosa
1.4.99.6 0.59
-
 D-arginine mutant A46G, pH 6.5, 25°C Pseudomonas aeruginosa
1.4.99.6 270
-
 D-arginine mutant S45A, pH 10.0, 25°C Pseudomonas aeruginosa
1.4.99.6 340
-
 D-arginine mutant A46G, pH 10.0, 25°C Pseudomonas aeruginosa