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Literature summary extracted from

  • Hartmann, T.; Schrapers, P.; Utesch, T.; Nimtz, M.; Rippers, Y.; Dau, H.; Mroginski, M.A.; Haumann, M.; Leimkuehler, S.
    The molybdenum active site of formate dehydrogenase is capable of catalyzing C-H bond cleavage and oxygen atom transfer reactions (2016), Biochemistry, 55, 2381-2389.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.9 C386S inactive Rhodobacter capsulatus
1.17.1.9 H387F inactive Rhodobacter capsulatus
1.17.1.9 H387K the mutant shows about 6200fold decreased catalytic efficiency compared to the wild type enzyme Rhodobacter capsulatus
1.17.1.9 H387M the mutant shows about 20fold decreased catalytic efficiency compared to the wild type enzyme Rhodobacter capsulatus
1.17.1.9 H387M/R587T inactive Rhodobacter capsulatus
1.17.1.9 H387R inactive Rhodobacter capsulatus
1.17.1.9 R587K the mutant shows about 6200fold decreased catalytic efficiency compared to the wild type enzyme Rhodobacter capsulatus
1.17.1.9 R587T inactive Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 iodoacetamide
-
 Rhodobacter capsulatus
1.17.1.9 nitrate competitive inhibitor Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.19
-
 formate wild type enzyme, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 3.6
-
 formate mutant enzyme H387M, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 27.5
-
 formate mutant enzyme H387K, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 362
-
 formate mutant enzyme R587K, at pH 9.0 and 25°C Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Rhodobacter capsulatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Rhodobacter capsulatus CO2 + NADH + H+
-
 ?
1.17.1.9 additional information the enzyme also exhibits nitrate reductase activity with methyl viologen as cosubstrate Rhodobacter capsulatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 Rhodobacter capsulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 0.9
-
 formate mutant enzyme H387K, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 11.2
-
 formate mutant enzyme R587K, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 33.7
-
 formate mutant enzyme H387M, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 35.4
-
 formate wild type enzyme, at pH 9.0 and 25°C Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 9
-
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 molybdenum cofactor
-
 Rhodobacter capsulatus
1.17.1.9 NAD+
-
 Rhodobacter capsulatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.1.9 1.6
-
 nitrate wild type enzyme, at pH 9.0 and 25°C Rhodobacter capsulatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.1.9 0.032
-
 formate mutant enzyme R587K, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 0.033
-
 formate mutant enzyme H387K, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 9.38
-
 formate mutant enzyme H387M, at pH 9.0 and 25°C Rhodobacter capsulatus
1.17.1.9 186.4
-
 formate wild type enzyme, at pH 9.0 and 25°C Rhodobacter capsulatus