EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.8 | phosphite dianion | strongly activates the wild-type enzyme. The N270A mutation results in a change in the effect of phosphite dianion on (kcat/Km)obs for GPDH-catalyzed reduction of glycerone phosphate, from strongly activating to inhibiting | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.8 | N270A | site-direted mutagenesis, the mutation results in a 7.7 kcal/mol decrease in the intrinsic phosphodianion binding energy, which is larger than the 5.6 kcal/mol effect of the mutation on the stability of the transition state for reduction of DHAP. A 2.2 kcal/mol stabilization of the transition state for unactivated hydride transfer to the truncated substrate glycolaldehyde, and a change in the effect of phosphite dianion on GPDH-catalyzed reduction of glycolaldehyde, from strongly activating to inhibiting. The N270A mutation breaks the network of hydrogen bonding side chains, Asn270, Thr264, Asn205, Lys204, Asp260, and Lys120, which connect the dianion activation and catalytic sites of GPDH. The disruption dramatically alters the performance of GPDH at these sites. The mutant enzyme shows highly decreased activity compared to the wild-type with glycerone phosphate,but 40fold higher activity with glycolaldehyde compared to the wild-type. There is no significant rescue of the activity of the N270A mutant with glycerone phosphate by 60 mM formamide | Homo sapiens |
1.1.1.8 | N270A | the mutant shows a strong decrease in catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.8 | R269A | site-direted mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type | Homo sapiens |
1.1.1.8 | R269A | the mutant shows a strong decrease in catalytic efficiency compared to the wild type enzyme | Homo sapiens |
1.1.1.8 | R269A/N270A | site-direted mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type. The N270A mutation at R269A hlGPDH results in the loss of the intrinsic side chain-dianion interaction | Homo sapiens |
1.1.1.8 | R269A/N270A | the mutant shows a strong decrease in catalytic efficiency compared to the wild type enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.8 | phosphite dianion | strongly inhibits the mutant N270A enzyme. The N270A mutation results in a change in the effect of phosphite dianion on (kcat/Km)obs for GPDH-catalyzed reduction of glycerone phosphate, from strongly activating to inhibiting | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.8 | additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
1.1.1.8 | 0.052 | - |
glycerone phosphate | wild-type enzyme, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 0.052 | - |
dihydroxyacetone phosphate | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 1.5 | - |
glycerone phosphate | mutant R269A/N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 2.5 | - |
glycerone phosphate | mutant N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 5.7 | - |
dihydroxyacetone phosphate | mutant enzyme R269A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 5.7 | - |
glycerone phosphate | mutant R269A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 15 | - |
dihydroxyacetone phosphate | mutant enzyme R269A/N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 25 | - |
dihydroxyacetone phosphate | mutant enzyme N270A, at pH 7.5 and 25°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.8 | glycerone phosphate + NADH + H+ | Homo sapiens | - |
sn-glycerol 3-phosphate + NAD+ | - |
? | |
1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Homo sapiens | - |
glycerone phosphate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.8 | Homo sapiens | - |
- |
- |
1.1.1.8 | Homo sapiens | P21695 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.8 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.8 | dihydroxyacetone phosphate + NADH + H+ | - |
Homo sapiens | ? | - |
? | |
1.1.1.8 | glycerone phosphate + NADH + H+ | - |
Homo sapiens | sn-glycerol 3-phosphate + NAD+ | - |
? | |
1.1.1.8 | glycerone phosphate + NADH + H+ | - |
Homo sapiens | sn-glycerol 3-phosphate + NAD+ | - |
r | |
1.1.1.8 | glycolaldehyde + NADH + H+ | - |
Homo sapiens | ? | - |
? | |
1.1.1.8 | glycolaldehyde + NADH + H+ | truncated substrate | Homo sapiens | ? + NAD+ | - |
r | |
1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Homo sapiens | glycerone phosphate + NADH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.8 | glycerol 3-phosphate dehydrogenase | - |
Homo sapiens |
1.1.1.8 | GPDH | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.8 | 25 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.8 | 0.00028 | - |
dihydroxyacetone phosphate | mutant enzyme R269A/N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.00028 | - |
glycerone phosphate | mutant R269A/N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 0.0059 | - |
dihydroxyacetone phosphate | mutant enzyme R269A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.0059 | - |
glycerone phosphate | mutant R269A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 9 | - |
dihydroxyacetone phosphate | mutant enzyme N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 9 | - |
glycerone phosphate | mutant N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 240 | - |
glycerone phosphate | wild-type enzyme, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 240 | - |
dihydroxyacetone phosphate | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.8 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.8 | NAD+ | - |
Homo sapiens | |
1.1.1.8 | NADH | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.8 | malfunction | mutation N270A results in a 7.7 kcal/mol decrease in the intrinsic phosphodianion binding energy, which is larger than the 5.6 kcal/mol effect of the mutation on the stability of the transition state for reduction of DHAP. A 2.2 kcal/mol stabilization of the transition state for unactivated hydride transfer to the truncated substrate glycolaldehyde, and a change in the effect of phosphite dianion on GPDH-catalyzed reduction of glycolaldehyde, from strongly activating to inhibiting. The N270A mutation breaks the network of hydrogen bonding side chains, Asn270, Thr264, Asn205, Lys204, Asp260, and Lys120, which connect the dianion activation and catalytic sites of GPDH. The disruption dramatically alters the performance of GPDH at these sites | Homo sapiens |
1.1.1.8 | additional information | structure-function analysis of wild-type and mutant enzymes, overview | Homo sapiens |
1.1.1.8 | physiological function | role for Asn270 in glycerol 3-phosphate dehydrogenase-catalyzed hydride transfer | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.8 | 0.000003 | - |
glycolaldehyde | mutant enzyme R269A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.000003 | - |
glycolaldehyde | mutant enzyme R269A/N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.00019 | - |
glycerone phosphate | mutant R269A/N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 0.001 | - |
dihydroxyacetone phosphate | mutant enzyme R269A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.001 | - |
glycerone phosphate | mutant R269A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 0.002 | - |
glycolaldehyde | mutant enzyme N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.17 | - |
dihydroxyacetone phosphate | mutant enzyme R269A/N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.36 | - |
dihydroxyacetone phosphate | mutant enzyme N270A, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 0.5 | - |
glycolaldehyde | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 3 | - |
glycolaldehyde | mutant R269A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 3 | - |
glycolaldehyde | mutant R269A/N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 3.6 | - |
glycerone phosphate | mutant N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 50 | - |
glycolaldehyde | wild-type enzyme, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 2000 | - |
glycolaldehyde | mutant N270A, pH 7.5, 25°C | Homo sapiens | |
1.1.1.8 | 4600 | - |
dihydroxyacetone phosphate | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
1.1.1.8 | 4615 | - |
glycerone phosphate | wild-type enzyme, pH 7.5, 25°C | Homo sapiens |