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Literature summary extracted from

  • Purcell, E.B.; McDonald, C.A.; Palfey, B.A.; Crosson, S.
    An analysis of the solution structure and signaling mechanism of LovK, a sensor histidine kinase integrating light and redox signals (2010), Biochemistry, 49, 6761-6770.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 gene CC_0285, recombinant expression of His-tagged wild-type enzyme, enzyme fragments, and mutant C70A enzyme in Escherichia coli strain Rosetta(DE3)pLysS Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 C70A site-directed mutagenesis Caulobacter vibrioides

General Stability

EC Number General Stability Organism
2.7.13.3 photoexcitation destabilizes the LOV-HK linker sequence Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.13.3 ATP + protein L-histidine Caulobacter vibrioides
-
 ADP + protein N-phospho-L-histidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Caulobacter vibrioides Q9ABE3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 recombinant His-tagged wild-type enzyme, enzyme fragments, and mutant C70A enzyme from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 ATP + protein L-histidine
-
 Caulobacter vibrioides ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 additional information measurement of the enzyme's ATPase activity Caulobacter vibrioides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.13.3 More LovK trypsin peptide mapping Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 CC_0285
-
 Caulobacter vibrioides
2.7.13.3 LovK
-
 Caulobacter vibrioides
2.7.13.3 sensor histidine kinase
-
 Caulobacter vibrioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.13.3 22
-
 assay at room temperature Caulobacter vibrioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.13.3 7.6
-
 assay at Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 ATP
-
 Caulobacter vibrioides
2.7.13.3 FMN bound to the LovK histidine kinase Caulobacter vibrioides
2.7.13.3 additional information no FAD Caulobacter vibrioides

General Information

EC Number General Information Comment Organism
2.7.13.3 additional information effect of the nonconserved flanking sequence on the signaling lifetime of LovK, the effects of structure outside the LOV core on its signaling lifetime do not combine in a simple, additive way. Kinetics of recovery from the adduct state depend not only on the buffer environment but also on the structural context in which the LOV domain is contained. Full-length LovK (residues 1-368) has a long recovery, with a half-life of 2 h, the isolated LOV core (residues 25-138) recovers more quickly, with a half-life of 37 min. LovK (25-163), which contains the LOV core and the nonconserved linker sequence at its C-terminus, has a recovery half-life of 28 min. A construct containing the LOV core and the nonconserved N-terminal flanking sequence, LovK(1-138), has a half-life of only 2 min, but this N-terminal region has not a general rate enhancement effect. LovK(1-163), a construct with fully intact N- and C-termini (but missing the kinase domain), is slower to recover than either LovK(25-138), LovK(1-138), or LovK(25-163) Caulobacter vibrioides