Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lountos, G.T.; Mitchell, K.H.; Studts, J.M.; Fox, B.G.; Orville, A.M.
    Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein (2005), Biochemistry, 44, 7131-7142.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.236 crystal structures for native T4moD and variants with either four (DELTAN4-), or 10 (DELTAN10-) residues removed from the N-terminal at 2.1-, 1.7-, and 1.9 A resolution, respectively. Alterations of the N-terminal have little influence on the folded core of the protein Pseudomonas mendocina

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.236 additional information construction of variants with either four (DELTAN4-) seven (DELTAN7-), or 10 (DELTAN10-) residues removed from the N-terminal. Removal leads to statistically insignificant changes in kcat, KM, kcat/KM, and KI relative to the native protein. There is no significant change in the regiospecificity of toluene oxidation with any of the T4moD variants Pseudomonas mendocina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.236 TmoD effector protein Pseudomonas mendocina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.236 0.0023
-
 Toluene mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.0032
-
 Toluene mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.0053
-
 Toluene mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.007
-
 Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q00459 subnit T4moD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96.2% 4-methylphenol, 1.5% 3-methylphenol, 0.9% 2-methylphenol, 1.4% benzyl alcohol ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.236 T4moD
-
 Pseudomonas mendocina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.236 2.8
-
 Toluene mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 3.1
-
 Toluene mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 3.3
-
 Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 3.4
-
 Toluene mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.236 0.098
-
 TmoD wild-type, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.106
-
 TmoD mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.11
-
 TmoD mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.116
-
 TmoD mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.236 470
-
 Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 530
-
 Toluene mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 970
-
 Toluene mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 1470
-
 Toluene mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina