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Literature summary extracted from

  • Pikus, J.D.; Studts, J.M.; McClay, K.; Steffan, R.J.; Fox, B.G.
    Changes in the regiospecificity of aromatic hydroxylation produced by active site engineering in the diiron enzyme toluene 4-monooxygenase (1997), Biochemistry, 36, 9283-9289.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.236 F205I decrease in regiospecificity for p-cresol formation, about 5-fold increase in the percentage of m-cresol formation. Mutant gives nearly equivalent amounts of benzylic and phenolic products from p-xylene oxidation Pseudomonas mendocina
1.14.13.236 Q141C decrease in regiospecificity for p-cresol formation, mutant functions predominantly as an aromatic ring hydroxylase during the oxidation of p-xylene Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q00456 tmoA polypeptide of the hydroxylase component
-
1.14.13.236 Pseudomonas mendocina KR1 Q00456 tmoA polypeptide of the hydroxylase component
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 2-xylene + NADH + H+ + O2
-
 Pseudomonas mendocina 3,4-dimethylphenol + NAD+ + H2O 95% 3,4-dimethylphenol plus 5% 2-methyl benzyl alcohol ?
1.14.13.236 2-xylene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 3,4-dimethylphenol + NAD+ + H2O 95% 3,4-dimethylphenol plus 5% 2-methyl benzyl alcohol ?
1.14.13.236 3-xylene + NADH + H+ + O2
-
 Pseudomonas mendocina 2,4-dimethylphenol + NAD+ + H2O 97% 2,4-dimethylphenol plus 2.5% 3-methyl benzyl alcohol plus 0.5% 3,5-dimethylphenol ?
1.14.13.236 3-xylene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 2,4-dimethylphenol + NAD+ + H2O 97% 2,4-dimethylphenol plus 2.5% 3-methyl benzyl alcohol plus 0.5% 3,5-dimethylphenol ?
1.14.13.236 4-xylene + NADH + H+ + O2
-
 Pseudomonas mendocina 2,5-dimethylphenol + 4-methyl benzyl alcohol + NAD+ + H2O 82% 2,5-dimethylphenol plus 18% 4-methyl benzyl alcohol ?
1.14.13.236 4-xylene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 2,5-dimethylphenol + 4-methyl benzyl alcohol + NAD+ + H2O 82% 2,5-dimethylphenol plus 18% 4-methyl benzyl alcohol ?
1.14.13.236 benzene + NADH + H+ + O2
-
 Pseudomonas mendocina phenol + NAD+ + H2O sole product ?
1.14.13.236 benzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 phenol + NAD+ + H2O sole product ?
1.14.13.236 chlorobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-chlorophenol + NAD+ + H2O more than 95% 4-chlorophenol ?
1.14.13.236 methoxybenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methoxyphenol + NAD+ + H2O more than 99% 4-methoxyphenol ?
1.14.13.236 additional information highly specific for para hydroxylation of toluene, o-xylene, m-xylene Pseudomonas mendocina ?
-
 ?
1.14.13.236 additional information highly specific for para hydroxylation of toluene, o-xylene, m-xylene Pseudomonas mendocina KR1 ?
-
 ?
1.14.13.236 nitrobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-nitrophenol + NAD+ + H2O 88% 4-nitrophenol plus 9% 3-nitrophenol plus 3% 2-nitrophenol ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol ?