BRENDA - Enzyme Database

Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties

Futai, M.; Biochemistry 12, 2468-2474 (1973)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.5.12
cyanide
stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.5.12
cyanide
stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH. At 1.5 mM, 50% inhibition at pH 9.3
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.12
0.6
-
(R)-lactate
pH 8.0, 23C
Escherichia coli
1.1.5.12
1.2
-
(R,S)-2-hydroxybutanoate
pH 8.0, 23C
Escherichia coli
1.1.5.12
1.4
-
(R)-lactate
presence of cyanide, pH 8.0, 23C
Escherichia coli
1.1.5.12
18
-
(S)-lactate
pH 8.0, 23C
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.12
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.12
71000
-
sucrose density gradient centrifugation
Escherichia coli
1.1.5.12
72000
-
1 * 72000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.12
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.12
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.1.5.12
-90C, 20% ethylene glycol, stable for at least 2 weeks
Escherichia coli
1.1.5.12
0C, 5 days, 80% residual activity
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.12
(R)-lactate + phenazine methosulfate
-
740028
Escherichia coli
pyruvate + reduced phenazine methosulfate
-
-
-
?
1.1.5.12
(R,S)-2-hydroxybutanoate + a quinone
12% of the activity with (R)-lactate
740028
Escherichia coli
2-oxobutanoate + a quinol
-
-
-
?
1.1.5.12
(S)-lactate + oxidized dichlorophenolindophenol
14% of the activity with (R)-lactate
740028
Escherichia coli
pyruvate + reduced dichlorophenolindophenol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.12
monomer
1 * 72000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.12
8
9
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.12
FAD
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.5.12
cyanide
stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.12
FAD
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.5.12
cyanide
stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH. At 1.5 mM, 50% inhibition at pH 9.3
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.12
0.6
-
(R)-lactate
pH 8.0, 23C
Escherichia coli
1.1.5.12
1.2
-
(R,S)-2-hydroxybutanoate
pH 8.0, 23C
Escherichia coli
1.1.5.12
1.4
-
(R)-lactate
presence of cyanide, pH 8.0, 23C
Escherichia coli
1.1.5.12
18
-
(S)-lactate
pH 8.0, 23C
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.12
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.12
71000
-
sucrose density gradient centrifugation
Escherichia coli
1.1.5.12
72000
-
1 * 72000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.12
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.5.12
-90C, 20% ethylene glycol, stable for at least 2 weeks
Escherichia coli
1.1.5.12
0C, 5 days, 80% residual activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.12
(R)-lactate + phenazine methosulfate
-
740028
Escherichia coli
pyruvate + reduced phenazine methosulfate
-
-
-
?
1.1.5.12
(R,S)-2-hydroxybutanoate + a quinone
12% of the activity with (R)-lactate
740028
Escherichia coli
2-oxobutanoate + a quinol
-
-
-
?
1.1.5.12
(S)-lactate + oxidized dichlorophenolindophenol
14% of the activity with (R)-lactate
740028
Escherichia coli
pyruvate + reduced dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.12
monomer
1 * 72000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.12
8
9
-
Escherichia coli