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Literature summary extracted from

  • Wang, X.; Xie, J.; da Mota, S.R.; Moore, C.E.; Proud, C.G.
    Regulated stability of eukaryotic elongation factor 2 kinase requires intrinsic but not ongoing activity (2015), Biochem. J., 467, 321-331.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin dependent on Homo sapiens

Application

EC Number Application Comment Organism
2.7.11.20 medicine the enzyme a possible target for therapeutic intervention in solid tumours Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ a Ca2+/calmodulin-dependent protein kinase Homo sapiens
2.7.11.20 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens O00418
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein autophosphorylation site is Thr348 in eEF2K Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 carcinoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 additional information the enzyme performs autophosphorylation on residue Thr348 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 eEF2K
-
 Homo sapiens
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.20 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.11.20 malfunction kinase-dead and other activity-deficient mutants of eEF2K are stabilized, as is a mutant lacking a critical autophosphorylation site (Thr348 in eEF2K), which is thought to be required for eEF2K and other alpha-kinases to achieve their active conformations. In contrast, application of small-molecule eEF2K inhibitors does not stabilize the protein. Achieving an active conformation, rather than eEF2K activity per se, is required for its susceptibility to degradation Homo sapiens
2.7.11.20 metabolism eEF2K is degraded by a proteasome-dependent pathway in response to genotoxic stress requiring a phosphodegron that includes an autophosphorylation site. The enzyme is also degraded under stress like including acidosis or treatment of cells with 2-deoxyglucose not requiring a phosphodegron Homo sapiens
2.7.11.20 physiological function eukaryotic elongation factor 2 kinase (eEF2K) is an atypical protein kinase which negatively regulates protein synthesis, is activated under stress conditions and plays a role in cytoprotection, e.g. in cancer cells Homo sapiens