EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.274 | synthesis | enzymatic production of the vitamin C precursor 2-keto-L-gulonate (2-KLG) from 2,5-diketo-D-gluconate (2,5-DKG) by coupling 2,5-diketo-D-gluconic acid reductase via its coenzyme to glucose dehydrogenase. The bienzymatic process shows complicated inhibition patterns caused by reaction products, NADP+ and NADPH. The key parameters for a fast and efficient conversion are the NADP(H) concentration, the volumetric activity of 2,5-DKG reductase, the ratio of synthetic enzyme activity to regenerate enzyme activity and the glucono-1,5-lactone concentration. By modeling the space-time yield of the process is nearly doubled and the coenzyme concentration reduced threefold | Corynebacterium glutamicum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.274 | expression in Escherichia coli | Corynebacterium glutamicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.274 | Corynebacterium glutamicum | H9CWC0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.274 | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
Corynebacterium glutamicum | 2-dehydro-D-gluconate + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.274 | Dkr | - |
Corynebacterium glutamicum |