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Literature summary extracted from

  • Jang, E.H.; Park, S.A.; Chi, Y.M.; Lee, K.S.
    Structural insight into the substrate inhibition mechanism of NADP(+)-dependent succinic semialdehyde dehydrogenase from Streptococcus pyogenes (2015), Biochem. Biophys. Res. Commun., 461, 487-493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.79
-
Streptococcus pyogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.79 structures in a binary complex with succinic semialdehyde as the substrate and a ternary complex with the substrate succinic semialdehyde and the inhibitory succinate semialdehyde, at 2.4 A resolution for both structures Streptococcus pyogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.79 succinate semialdehyde uncompetitive substrate inhibition above 0.02 mM, in the presence of NADP+. Substrate inhibition is induced by the binding of inhibitory succinate semialdehyde in the cofactor-binding site, instead of NADP+ Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.79 3.95
-
succinate semialdehyde pH 7.0, 30°C Streptococcus pyogenes

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.79 Streptococcus pyogenes A0A0J9X1M8
-
-
1.2.1.79 Streptococcus pyogenes MGAS1882 A0A0J9X1M8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
Streptococcus pyogenes succinate + NADPH + 2 H+
-
?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
Streptococcus pyogenes MGAS1882 succinate + NADPH + 2 H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.79 gabD
-
Streptococcus pyogenes
1.2.1.79 succinic semialdehyde dehydrogenase
-
Streptococcus pyogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.79 1.69
-
succinate semialdehyde pH 7.0, 30°C Streptococcus pyogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.79 NADP+
-
Streptococcus pyogenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.79 0.1
-
succinate semialdehyde pH 7.0, 30°C Streptococcus pyogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.79 0.43
-
succinate semialdehyde pH 7.0, 30°C Streptococcus pyogenes