EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.4 | expressed in Escherichia coli CodonPlus (DE3)-RIPL cells | Pyrobaculum ferrireducens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.4 | apoenzyme and in complex with isobutyraldehyde and/or NADP+, capillary counterdiffusion method | Pyrobaculum ferrireducens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.4 | 15 | - |
glyceraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
1.2.1.4 | 20 | - |
Isobutyraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
1.2.1.4 | 21 | - |
propionaldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.4 | Pyrobaculum ferrireducens | G7VCG0 | - |
- |
1.2.1.4 | Pyrobaculum ferrireducens 1860 | G7VCG0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.4 | Source 30Q column chromatography and Superdex 200 gel filtration | Pyrobaculum ferrireducens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.4 | glyceraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens | glycerate + NADPH + H+ | - |
ir | |
1.2.1.4 | glyceraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens 1860 | glycerate + NADPH + H+ | - |
ir | |
1.2.1.4 | isobutyraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens | isobutyrate + NADPH + H+ | - |
ir | |
1.2.1.4 | isobutyraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens 1860 | isobutyrate + NADPH + H+ | - |
ir | |
1.2.1.4 | additional information | no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde | Pyrobaculum ferrireducens | ? | - |
? | |
1.2.1.4 | additional information | no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde | Pyrobaculum ferrireducens 1860 | ? | - |
? | |
1.2.1.4 | propionaldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens | propanoate + NADPH + H+ | - |
ir | |
1.2.1.4 | propionaldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens 1860 | propanoate + NADPH + H+ | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.4 | AlDHPyr1147 | - |
Pyrobaculum ferrireducens |
1.2.1.4 | NADP-dependent aldehyde dehydrogenase | - |
Pyrobaculum ferrireducens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.4 | 75 | - |
- |
Pyrobaculum ferrireducens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.4 | 60 | 85 | - |
Pyrobaculum ferrireducens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.4 | 1.2 | - |
glyceraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
1.2.1.4 | 2.9 | - |
propionaldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
1.2.1.4 | 4.1 | - |
Isobutyraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.4 | 8.5 | 9.5 | - |
Pyrobaculum ferrireducens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.4 | NADP+ | the enzyme is strictly specific for NADP+ | Pyrobaculum ferrireducens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.4 | 0.08 | - |
glyceraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
1.2.1.4 | 0.14 | - |
propionaldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
1.2.1.4 | 0.2 | - |
Isobutyraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens |