Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dudzik, A.; Snoch, W.; Borowiecki, P.; Opalinska-Piskorz, J.; Witko, M.; Heider, J.; Szaleniec, M.
    Asymmetric reduction of ketones and beta-keto esters by (S)-1-phenylethanol dehydrogenase from denitrifying bacterium Aromatoleum aromaticum (2015), Appl. Microbiol. Biotechnol., 99, 5055-5069.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.311 synthesis application of (S)-1-phenylethanol dehydrogenase for asymmetric reduction of 42 prochiral ketones and 11 beta-keto esters to enantiopure secondary alcohols. The conversions are carried out in a batch reactor using recombinant Escherichia coli as whole-cell catalysts and isopropanol as reaction solvent and cosubstrate for NADH recovery. Ketones are converted to the respective secondary alcohols with excellent enantiomeric excesses and high productivities Aromatoleum aromaticum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.311 expression in Escherichia coli Aromatoleum aromaticum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.311 docking simulation for prochiral ketone and beta-keto ester substrates Aromatoleum aromaticum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.311 Aromatoleum aromaticum Q5P5I4
-
-
1.1.1.311 Aromatoleum aromaticum EbN1 Q5P5I4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.311 2,2,2-trifluoroacetophenone + NADH + H+
-
 Aromatoleum aromaticum (S)-1-phenyl-2,2,2-trifluoroethan-1-ol + NAD+ 100% enantiomertic excess ?
1.1.1.311 2,2,2-trifluoroacetophenone + NADH + H+
-
 Aromatoleum aromaticum EbN1 (S)-1-phenyl-2,2,2-trifluoroethan-1-ol + NAD+ 100% enantiomertic excess ?
1.1.1.311 2,4'-dichloroacetophenone + NADH + H+
-
 Aromatoleum aromaticum (S)-2-chloro-1-(4-chloro-phenyl)-ethanol + NAD+ 100% enantiomeric excess ?
1.1.1.311 2,4'-dichloroacetophenone + NADH + H+
-
 Aromatoleum aromaticum EbN1 (S)-2-chloro-1-(4-chloro-phenyl)-ethanol + NAD+ 100% enantiomeric excess ?
1.1.1.311 4'-hydroxyacetophenone + NADH + H+
-
 Aromatoleum aromaticum (S)-4-(1-hydroxyethyl)phenol + NAD+ 90% enantiomeric excess ?
1.1.1.311 4-acetylbiphenyl + NADH + H+
-
 Aromatoleum aromaticum (S)-1-(biphenyl-4-yl)ethanol + NAD+ 100% enantiomeric excess ?
1.1.1.311 4-acetylbiphenyl + NADH + H+
-
 Aromatoleum aromaticum EbN1 (S)-1-(biphenyl-4-yl)ethanol + NAD+ 100% enantiomeric excess ?
1.1.1.311 4-aminoacetophenone + NADH + H+
-
 Aromatoleum aromaticum 1-(4-aminophenyl)ethanol + NAD+ racemic product ?
1.1.1.311 4-aminoacetophenone + NADH + H+
-
 Aromatoleum aromaticum EbN1 1-(4-aminophenyl)ethanol + NAD+ racemic product ?
1.1.1.311 acetophenone + NADH + H+
-
 Aromatoleum aromaticum (S)-1-phenylethanol + NAD+ 100% enantiomeric excess ?
1.1.1.311 acetophenone + NADH + H+
-
 Aromatoleum aromaticum EbN1 (S)-1-phenylethanol + NAD+ 100% enantiomeric excess ?
1.1.1.311 methyl 4-fluorobenzoylacetate + NADH + H+
-
 Aromatoleum aromaticum (S)-methyl 3-(4-fluorophenyl)-3-hydroxypropanoate + NAD+ 100% enantiomertic excess ?