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Literature summary extracted from

  • You, Z.Y.; Liu, Z.Q.; Zheng, Y.G.
    Characterization of a newly synthesized carbonyl reductase and construction of a biocatalytic process for the synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate with high space-time yield (2014), Appl. Microbiol. Biotechnol., 98, 1671-1680.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.B3 additional information not inhibitory or activating: EDTA, dithiothreitol Candida parapsilosis
1.1.1.B3 Triton X-100 1%, 112% of initial activity Candida parapsilosis
1.1.1.B3 Tween-20 1%, 111% of initial activity Candida parapsilosis
1.1.1.B3 Tween-80 1%, 115% of initial activity Candida parapsilosis

Application

EC Number Application Comment Organism
1.1.1.B3 synthesis using recombinant Scr2 in an aqueous-organic solvent system with a substrate fed-batch strategy and a final substrate concentration of 1 M, a yield of 95.3% and e.e. of 99% is obtained after 6-h reaction Candida parapsilosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B3 Ag+ 1 mM, no residual activity Candida parapsilosis
1.1.1.B3 Cu2+ 1 mM, no residual activity Candida parapsilosis
1.1.1.B3 Fe2+ 1 mM, 2.5% residual activity Candida parapsilosis
1.1.1.B3 Fe3+ 1 mM, 44% residual activity Candida parapsilosis
1.1.1.B3 Mg2+ 1 mM, 77% residual activity Candida parapsilosis
1.1.1.B3 additional information not inhibitory or activating: EDTA, dithiothreitol Candida parapsilosis
1.1.1.B3 SDS 1%, no residual activity Candida parapsilosis
1.1.1.B3 Zn2+ 1 mM, 79% residual activity Candida parapsilosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B3 6.4
-
 4-chloro-3-oxobutanoate pH 6.0, 45°C Candida parapsilosis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.B3 benzene 85% residual activity Candida parapsilosis
1.1.1.B3 cyclohexane 88% residual activity Candida parapsilosis
1.1.1.B3 dimethyl formamide 5%, 82% residual activity Candida parapsilosis
1.1.1.B3 dimethyl phthallate 131% residual activity Candida parapsilosis
1.1.1.B3 dimethyl sulfoxide 5%, 89% residual activity Candida parapsilosis
1.1.1.B3 heptane 101% residual activity Candida parapsilosis
1.1.1.B3 hexane 74% residual activity Candida parapsilosis
1.1.1.B3 isooctane 106% residual activity Candida parapsilosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B3 Candida parapsilosis D5G304
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B3 2,3-butanedione + NADPH + H+
-
 Candida parapsilosis ? + NADP+
-
 ?
1.1.1.B3 4-chloro-3-oxobutanoate + NADH + H+
-
 Candida parapsilosis (S)-4-chloro-3-hydroxybutanoate + NAD+ 99% enantiomeric excess of (S)-enantiomer ?
1.1.1.B3 ethyl 3-oxobutanoate + NADPH + H+
-
 Candida parapsilosis ethyl (S)-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.B3 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Candida parapsilosis ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.B3 methyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Candida parapsilosis methyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.B3 additional information no substrates: cetophenone, 2-hydroxyacetophenone, acetone, butanone, 2-pentanone, 3-pentanone, 2-hexanone, 2-heptanone, 2-octanone, cyclohexanone, acetylacetone, 2,5-hexanedione and 5,5-dimethyl-l,3-cyclohexanedione Candida parapsilosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B3 Scr2
-
 Candida parapsilosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B3 45
-
-
 Candida parapsilosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.B3 30
-
 half-life 16.5 h Candida parapsilosis
1.1.1.B3 40
-
 half-life 2.2 h Candida parapsilosis
1.1.1.B3 50
-
 half-life 0.05 h Candida parapsilosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B3 6
-
-
 Candida parapsilosis