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Literature summary extracted from

  • Chen, C.; Joo, J.C.; Brown, G.; Stolnikova, E.; Halavaty, A.S.; Savchenko, A.; Anderson, W.F.; Yakunin, A.F.
    Structure-based mutational studies of substrate inhibition of betaine aldehyde dehydrogenase BetB from Staphylococcus aureus (2014), Appl. Environ. Microbiol., 80, 3992-4002.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 expressed in Escherichia coli BL21(DE3) Gold cells Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 G234A the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 G234S the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 G234T the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 H448F the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 H448F/P449M the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 H448F/P449M/Y450L the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 H448F/Y450L the mutant demonstrates a complete loss of substrate inhibition Staphylococcus aureus
1.2.1.8 L161M/Q162M the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 P449M/Y450L the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 Q162M the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 S290T the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 V288D the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 W456H the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
1.2.1.8 Y450L the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 Betaine aldehyde substrate inhibition at concentrations of betaine aldehyde as low as 0.15 mM Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.11
-
 Betaine aldehyde mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.12
-
 Betaine aldehyde mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.12
-
 NAD+ mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.17
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.17
-
 Betaine aldehyde mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.26
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.26
-
 Betaine aldehyde mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.27
-
 NAD+ mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.3
-
 NAD+ mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.34
-
 NAD+ mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.38
-
 NAD+ mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.41
-
 NAD+ mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.42
-
 NAD+ mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.43
-
 NAD+ mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.57
-
 Betaine aldehyde mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.68
-
 NAD+ mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.71
-
 NAD+ mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.91
-
 Betaine aldehyde mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.01
-
 NAD+ mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.12
-
 NAD+ mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.12
-
 NAD+ mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.18
-
 Betaine aldehyde mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.92
-
 Betaine aldehyde mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.94
-
 Betaine aldehyde mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 2.16
-
 Betaine aldehyde mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 3.42
-
 NAD+ mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 4.61
-
 Betaine aldehyde mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 4.76
-
 Betaine aldehyde mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 13.8
-
 Betaine aldehyde mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 14.8
-
 Betaine aldehyde mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Staphylococcus aureus
-
 betaine + NADH + 2 H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Staphylococcus aureus betaine + NADH + 2 H+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
 Staphylococcus aureus
1.2.1.8 BetB
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.8 0.1
-
 Betaine aldehyde mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.25
-
 Betaine aldehyde mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.94
-
 Betaine aldehyde mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.09
-
 Betaine aldehyde mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.14
-
 Betaine aldehyde mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.35
-
 Betaine aldehyde mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.63
-
 Betaine aldehyde mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.8
-
 Betaine aldehyde mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 1.87
-
 Betaine aldehyde mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 2.92
-
 Betaine aldehyde mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 3.2
-
 Betaine aldehyde mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 4.06
-
 Betaine aldehyde mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 4.46
-
 Betaine aldehyde mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 9.9
-
 Betaine aldehyde mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 11
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+ the enzyme can use both NAD+ and NADP+ as cofactors, but the NAD+-dependent activity is at least 10 times higher Staphylococcus aureus
1.2.1.8 NADP+ the enzyme can use both NAD+ and NADP+ as cofactors, but the NAD+-dependent activity is at least 10 times higher Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.8 0.34
-
 Betaine aldehyde pH and temperature not specified in the publication Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.8 0.053
-
 Betaine aldehyde mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.12
-
 Betaine aldehyde mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.22
-
 Betaine aldehyde mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.39
-
 Betaine aldehyde mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.7
-
 Betaine aldehyde mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.91
-
 Betaine aldehyde mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.92
-
 Betaine aldehyde mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.96
-
 Betaine aldehyde mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 2.06
-
 Betaine aldehyde mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 3.62
-
 Betaine aldehyde mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 7.12
-
 Betaine aldehyde mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 9.5
-
 Betaine aldehyde mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 10.9
-
 Betaine aldehyde mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 17.2
-
 Betaine aldehyde mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 64.7
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus