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Literature summary extracted from

  • Shibasaki, T.; Mori, H.; Chiba, S.; Ozaki, A.
    Microbial proline 4-hydroxylase screening and gene cloning (1999), Appl. Environ. Microbiol., 65, 4028-4031.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.57 expression in Escherichia coli Dactylosporangium sp. RH1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.57 29715
-
-
 Dactylosporangium sp. RH1
1.14.11.57 31000
-
 gel filtration Dactylosporangium sp. RH1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.57 L-proline + 2-oxoglutarate + O2 Dactylosporangium sp. RH1
-
 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.57 Dactylosporangium sp. RH1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.57 partial Dactylosporangium sp. RH1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.57 2080
-
 pH 6.5, 35°C Dactylosporangium sp. RH1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.57 L-proline + 2-oxoglutarate + O2
-
 Dactylosporangium sp. RH1 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.57 monomer 1 * 31000, SDS-PAGE, 1 * 29715, calculated Dactylosporangium sp. RH1