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Literature summary extracted from

  • Li, A.; Ilie, A.; Sun, Z.; Lonsdale, R.; Xu, J.; Reetz, M.
    Whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution (2016), Angew. Chem. Int. Ed. Engl., 55, 12026-12029.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.174 functional recombinant expression in Escherichia coli Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.174 L181F random mutagenesis, A site mutant Priestia megaterium
1.1.1.174 L181F/I263N random mutagenesis, A site mutant Priestia megaterium
1.1.1.174 additional information whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution: in Escherichia coli, expression of the recombinant enzyme leads to four different mutant whole cells, each leading to one of the three possible stereoisomeric forms of 1,2-cyclohexanediol, namely (R,R)-5, (S,S)-5, (S,R)-5 (meso), or (R,S)-5 (also meso), in a multi-step sequence, overview. Starting from the achiral substrates cyclohexane, cyclohexanol, or cyclohexanone, cyclohexane-1,2-diols as functionalized products are shown to be accessible in three different stereoisomeric forms Priestia megaterium
1.1.1.174 V78I/A82F/A328F/T438I random mutagenesis, B site mutant Priestia megaterium
1.1.1.174 V78I/A82I/A328N random mutagenesis, B site mutant Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.174 trans-cyclohexane-1,2-diol + NAD+ Priestia megaterium
-
 2-hydroxycyclohexan-1-one + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.174 Priestia megaterium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.174 trans-cyclohexane-1,2-diol + NAD+
-
 Priestia megaterium 2-hydroxycyclohexan-1-one + NADH + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.174 NAD+
-
 Priestia megaterium