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Literature summary extracted from

  • Wettstein, C.; Kano, K.; Schäfer, D.; Wollenberger, U.; Lisdat, F.
    Interaction of flavin-dependent fructose dehydrogenase with cytochrome c as basis for the construction of biomacromolecular architectures on electrodes (2016), Anal. Chem., 88, 6382-6389.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.14 2.6
-
 D-fructose at pH 5.0 and 37°C Gluconobacter japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.14 19700
-
-
 Gluconobacter japonicus
1.1.5.14 50800
-
-
 Gluconobacter japonicus
1.1.5.14 67000
-
-
 Gluconobacter japonicus
1.1.5.14 140000
-
 gel filtration Gluconobacter japonicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.14 Gluconobacter japonicus
-
 formerly Gluconobacter industrius
-
1.1.5.14 Gluconobacter japonicus NBRCA3260
-
 formerly Gluconobacter industrius
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.14 D-fructose + ferricyanide
-
 Gluconobacter japonicus 5-dehydro-D-fructose + ferrocyanide
-
 ?
1.1.5.14 D-fructose + ferricyanide
-
 Gluconobacter japonicus NBRCA3260 5-dehydro-D-fructose + ferrocyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.14 heterotrimer 1 * 67000 + 1 * 50800 + 1 * 19700, calculated from amino acid sequence Gluconobacter japonicus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.14 FDH
-
 Gluconobacter japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.14 37
-
-
 Gluconobacter japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.14 4 4.5
-
 Gluconobacter japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.14 FAD
-
 Gluconobacter japonicus
1.1.5.14 heme c
-
 Gluconobacter japonicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.5.14 Gluconobacter japonicus isoelectric focusing
-
 5