Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Furukawa, N.; Miyanaga, A.; Togawa, M.; Nakajima, M.; Taguchi, H.
    Diverse allosteric and catalytic functions of tetrameric D-lactate dehydrogenases from three Gram-negative bacteria (2014), AMB Express, 4, 76.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.28 fructose 1,6-bisphosphate required Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 fructose 1,6-bisphosphate required Pseudomonas aeruginosa
1.1.1.28 additional information no activation of the enzyme by fructose 1,6-bisphosphate and Mg2+ Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 gene ecd_01352, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Escherichia coli
1.1.1.28 gene fn0511, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 gene pa0927, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 glyoxylate
-
Pseudomonas aeruginosa
1.1.1.28 Hydroxypyruvate
-
Pseudomonas aeruginosa
1.1.1.28 oxaloacetate
-
Escherichia coli
1.1.1.28 oxaloacetate
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 oxamate constant inhibition at pH 7.0 and pH 8.0 Escherichia coli
1.1.1.28 oxamate inhibits the catalytic reaction of FNLDH competitively with pyruvate, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 oxamate inhibits the catalytic reaction of PALDH in a mixed--type manner at pH 7.0, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 Pseudomonas aeruginosa
1.1.1.28 phenylpyruvate
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 pyruvate
-
Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 additional information
-
additional information kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows negative cooperativity in the pyruvate saturation curve above pH 7.5 Pseudomonas aeruginosa
1.1.1.28 additional information
-
additional information kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 additional information
-
additional information kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 Escherichia coli
1.1.1.28 0.019
-
NADH pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 0.022
-
NADH pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.028
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.031
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.035
-
NADH pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ Pseudomonas aeruginosa
1.1.1.28 0.036
-
NADH pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.055
-
NADH pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 0.088
-
NADH pH 8.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 0.09
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
1.1.1.28 0.092
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
1.1.1.28 0.1
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 0.31
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.34
-
pyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.36
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
1.1.1.28 0.64
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 0.68
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 0.85
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
1.1.1.28 1.2
-
pyruvate pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 1.2
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 1.7
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 1.7
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 2.5
-
pyruvate pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 2.5
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 2.6
-
pyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 2.6
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 3
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 3.5
-
2-oxoisovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 3.8
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 4
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 5.8
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 6.5
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
1.1.1.28 6.8
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 7.1
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 7.2
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
1.1.1.28 7.6
-
pyruvate pH 8.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 11
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 12
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 12
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 18
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 18
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 20
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 30
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 54
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.28 Mg2+ activates Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 Mg2+ activates Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 37800
-
-
Pseudomonas aeruginosa
1.1.1.28 38500
-
-
Escherichia coli
1.1.1.28 40000
-
4 * 40000, about, sequence calculation Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 120000
-
recombinant His-tagged enzyme, gel filtration Pseudomonas aeruginosa
1.1.1.28 150000
-
recombinant His-tagged enzyme, peak 2, gel filtration Escherichia coli
1.1.1.28 160000
-
recombinant His-tagged enzyme, gel filtration Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 330000
-
recombinant His-tagged enzyme, peak 1, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Fusobacterium nucleatum subsp. nucleatum
-
pyruvate + NADH + H+
-
r
1.1.1.28 (R)-lactate + NAD+ Escherichia coli
-
pyruvate + NADH + H+
-
r
1.1.1.28 pyruvate + NADH + H+ Pseudomonas aeruginosa
-
(R)-lactate + NAD+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Escherichia coli A0A140N893
-
-
1.1.1.28 Fusobacterium nucleatum subsp. nucleatum Q8RG11 strain JCM 8532
-
1.1.1.28 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 Q8RG11 strain JCM 8532
-
1.1.1.28 Pseudomonas aeruginosa Q9I530
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration Pseudomonas aeruginosa
1.1.1.28 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.28 (R)-lactate + NAD+ = pyruvate + NADH + H+ allosteric mechanism Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 (R)-lactate + NAD+ = pyruvate + NADH + H+ allosteric mechanism Pseudomonas aeruginosa
1.1.1.28 (R)-lactate + NAD+ = pyruvate + NADH + H+ allosteric mechanism Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
Fusobacterium nucleatum subsp. nucleatum pyruvate + NADH + H+
-
r
1.1.1.28 (R)-lactate + NAD+
-
Escherichia coli pyruvate + NADH + H+
-
r
1.1.1.28 2-oxobutyrate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxybutyrate + NAD+
-
r
1.1.1.28 2-oxobutyrate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxybutyrate + NAD+
-
r
1.1.1.28 2-oxobutyrate + NADH + H+
-
Escherichia coli 2-hydroxybutyrate + NAD+
-
r
1.1.1.28 2-oxobutyrate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 2-hydroxybutyrate + NAD+
-
r
1.1.1.28 2-oxocaproate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxycaproate + NAD+
-
r
1.1.1.28 2-oxocaproate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxycaproate + NAD+
-
r
1.1.1.28 2-oxocaproate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 2-hydroxycaproate + NAD+
-
r
1.1.1.28 2-oxoisocaproate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxyisocaproate + NAD+
-
r
1.1.1.28 2-oxoisocaproate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxyisocaproate + NAD+
-
r
1.1.1.28 2-oxoisovalerate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxyisovalerate + NAD+
-
r
1.1.1.28 2-oxovalerate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxyvalerate + NAD+
-
r
1.1.1.28 2-oxovalerate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxyvalerate + NAD+
-
r
1.1.1.28 glyoxylate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum hydroxyacetate + NAD+
-
r
1.1.1.28 glyoxylate + NADH + H+
-
Pseudomonas aeruginosa hydroxyacetate + NAD+
-
r
1.1.1.28 glyoxylate + NADH + H+
-
Escherichia coli hydroxyacetate + NAD+
-
r
1.1.1.28 hydroxypyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum glycerate + NAD+
-
r
1.1.1.28 hydroxypyruvate + NADH + H+
-
Pseudomonas aeruginosa glycerate + NAD+
-
r
1.1.1.28 hydroxypyruvate + NADH + H+
-
Escherichia coli glycerate + NAD+
-
r
1.1.1.28 hydroxypyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 glycerate + NAD+
-
r
1.1.1.28 additional information the enzyme ECLDH exhibits high substrate specificity toward pyruvate. No or poor activity with 2-oxovalerate, 2-oxoisovalerate, 2-oxocaproate, 2-oxoisocaproate, phenylpyruvate, and hydroxyphenylpyruvate Escherichia coli ?
-
?
1.1.1.28 additional information the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate Fusobacterium nucleatum subsp. nucleatum ?
-
?
1.1.1.28 additional information the enzyme PALDH exhibits high substrate specificity toward pyruvate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate Pseudomonas aeruginosa ?
-
?
1.1.1.28 additional information the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 ?
-
?
1.1.1.28 oxaloacetate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum malate + NAD+
-
r
1.1.1.28 oxaloacetate + NADH + H+
-
Pseudomonas aeruginosa malate + NAD+
-
r
1.1.1.28 oxaloacetate + NADH + H+
-
Escherichia coli malate + NAD+
-
r
1.1.1.28 phenylpyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum D-phenyllactate + NAD+
-
r
1.1.1.28 phenylpyruvate + NADH + H+
-
Pseudomonas aeruginosa D-phenyllactate + NAD+
-
r
1.1.1.28 pyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum (R)-lactate + NAD+
-
r
1.1.1.28 pyruvate + NADH + H+
-
Pseudomonas aeruginosa (R)-lactate + NAD+
-
r
1.1.1.28 pyruvate + NADH + H+
-
Escherichia coli (R)-lactate + NAD+
-
r
1.1.1.28 pyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 (R)-lactate + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.28 homooctamer 8 * 38500, about, sequence calculation, the apparently octameric form of ECLDH is likely an artificial product due to the overexpression, and exhibits slightly but significantly lower specific activity than the tetrameric form Escherichia coli
1.1.1.28 homotetramer 4 * 40000, about, sequence calculation Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 homotetramer 4 * 38500, about, sequence calculation Escherichia coli
1.1.1.28 homotrimer 4 * 37800, about, sequence calculation Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-LDH
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 D-LDH
-
Pseudomonas aeruginosa
1.1.1.28 D-LDH
-
Escherichia coli
1.1.1.28 ECBD_2243
-
Escherichia coli
1.1.1.28 ECLDH
-
Escherichia coli
1.1.1.28 FN0511
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 FNLDH
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 PA0927
-
Pseudomonas aeruginosa
1.1.1.28 PALDH
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 30
-
assay at Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 30
-
assay at Pseudomonas aeruginosa
1.1.1.28 30
-
assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.28 39
-
purified recombinant His-tagged enzyme, pH 8.0, stable up to Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 49
-
purified recombinant His-tagged enzyme, pH 8.0, stable up to Escherichia coli
1.1.1.28 57
-
purified recombinant His-tagged enzyme, pH 5.0, stable up to Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 11
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 18
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 31
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 39
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 50
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 69
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 80
-
pyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 80
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 80
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 80
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 87
-
pyruvate pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 90
-
NADH pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 98
-
NADH pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 100
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 100
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 100
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 110
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 120
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 120
-
2-oxoisovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 140
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 140
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 160
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 160
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 200
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 230
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 240
-
pyruvate pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 240
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
1.1.1.28 270
-
NADH pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ Pseudomonas aeruginosa
1.1.1.28 290
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
1.1.1.28 310
-
NADH pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 370
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
1.1.1.28 380
-
pyruvate pH 8.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 400
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 400
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
1.1.1.28 410
-
pyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 410
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 420
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 640
-
NADH pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 690
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
1.1.1.28 710
-
NADH pH 8.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 730
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
1.1.1.28 880
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 1100
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 7 8 assay at Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 7 8 assay at Pseudomonas aeruginosa
1.1.1.28 7 8 assay at Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.28 4 8 purified recombinant His-tagged enzyme, 30°C, 1 h, stable at Pseudomonas aeruginosa
1.1.1.28 5 10 purified recombinant His-tagged enzyme, 30°C, 1 h, stable at Escherichia coli
1.1.1.28 5 8.5 purified recombinant His-tagged enzyme, 30°C, 1 h, stable at Fusobacterium nucleatum subsp. nucleatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 NAD+
-
Pseudomonas aeruginosa
1.1.1.28 NAD+
-
Escherichia coli
1.1.1.28 NADH
-
Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 NADH
-
Pseudomonas aeruginosa
1.1.1.28 NADH
-
Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.28 additional information
-
additional information inhibition kinetics Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 additional information
-
additional information inhibition kinetics Pseudomonas aeruginosa
1.1.1.28 additional information
-
additional information inhibition kinetics Escherichia coli
1.1.1.28 1.8
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 5
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 19
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 20
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
1.1.1.28 23
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 44
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 140
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum

General Information

EC Number General Information Comment Organism
1.1.1.28 evolution although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 evolution although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview Pseudomonas aeruginosa
1.1.1.28 evolution although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview Escherichia coli
1.1.1.28 metabolism the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 metabolism the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions Pseudomonas aeruginosa
1.1.1.28 metabolism the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.28 0.7
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 1
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 2.6
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 3.2
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 4.2
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 5.3
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 8.7
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 11
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 18
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 21
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 27
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 31
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 35
-
pyruvate pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 35
-
2-oxoisovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 50
-
pyruvate pH 8.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 51
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
1.1.1.28 53
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 60
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 61
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
1.1.1.28 65
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 87
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 130
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 140
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 150
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 160
-
pyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 200
-
pyruvate pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 200
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 230
-
pyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 230
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 280
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
1.1.1.28 440
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 820
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
1.1.1.28 2500
-
NADH pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 2800
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 3700
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 4000
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 4400
-
NADH pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1.1.1.28 7700
-
NADH pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.1.1.28 7700
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
1.1.1.28 7700
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
1.1.1.28 7800
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
1.1.1.28 8000
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
1.1.1.28 8100
-
NADH pH 8.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 12000
-
NADH pH 7.0, 30°C, recombinant enzyme Escherichia coli
1.1.1.28 17000
-
NADH pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa