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Literature summary extracted from

  • Usui, T.; Okada, M.; Hara, Y.; Yamawaki, H.
    Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation (2013), Am. J. Physiol. Heart Circ. Physiol., 305, H756-H768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin a central mediator of Ca2+-dependent signaling, the enzyme is dependent on calmodulin Homo sapiens
2.7.11.20 Calmodulin a central mediator of Ca2+-dependent signaling, the enzyme is dependent on calmodulin Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.20 additional information generation of enzyme knoockout mice, silencing of the enzyme by siRNA expression Rattus norvegicus
2.7.11.20 additional information knockdown of eEF2K gene by small interfering RNA (siRNA) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.20 1-benzyl-3-cetyl-2-methylimidazolium iodide i.e. NH125, inhibits eEF2K activity via inhibition of alpha-kinase, a catalytic domain of eEF2K Homo sapiens
2.7.11.20 1-benzyl-3-cetyl-2-methylimidazolium iodide i.e. NH125, inhibits eEF2K activity via inhibition of alpha-kinase, a catalytic domain of eEF2K. In mesenteric artery from spontaneously hypertensive rats, the acetylcholine-induced endothelium-dependent relaxation is significantly impaired after long-term treatment with the inhibitor Rattus norvegicus
2.7.11.20 7-amino-1-cyclopropyl-3-ethyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d] pyrimidine-6-carboxamide i.e. A-484954, a pyrido-pyrimidinedione derivative that inhibits eEF2K in an ATP-competitive but CaM-independent manner Homo sapiens
2.7.11.20 7-amino-1-cyclopropyl-3-ethyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d] pyrimidine-6-carboxamide i.e. A-484954, a pyrido-pyrimidinedione derivative that inhibits eEF2K in an ATP-competitive but CaM-independent manner Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ a Ca2+/calmodulin-dependent protein kinase Homo sapiens
2.7.11.20 Ca2+ a Ca2+/calmodulin-dependent protein kinase Rattus norvegicus
2.7.11.20 Mg2+ required Homo sapiens
2.7.11.20 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2] Rattus norvegicus
-
 ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2] Rattus norvegicus Wistar
-
 ADP + [elongation factor 2] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens O00418
-
-
2.7.11.20 Rattus norvegicus P70531
-
-
2.7.11.20 Rattus norvegicus Wistar P70531
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 additional information activity of eEF2K is selectively increased in proliferating cells Homo sapiens
-
2.7.11.20 additional information activity of eEF2K is selectively increased in proliferating cells Rattus norvegicus
-
2.7.11.20 U-937 cell
-
 Homo sapiens
-
2.7.11.20 umbilical vein endothelial cell
-
 Homo sapiens
-
2.7.11.20 vascular endothelial cell
-
 Rattus norvegicus
-
2.7.11.20 vascular smooth muscle cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2]
-
 Rattus norvegicus ADP + [elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2]
-
 Rattus norvegicus Wistar ADP + [elongation factor 2] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 eEF2K
-
 Homo sapiens
2.7.11.20 eEF2K
-
 Rattus norvegicus
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Homo sapiens
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.20 ATP
-
 Homo sapiens
2.7.11.20 ATP
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.11.20 malfunction eEF2K gene knockdown on TNF-alpha-induced inflammatory responses in HUVECs Homo sapiens
2.7.11.20 malfunction enzyme silencing with eEF2K siRNA inhibits phosphorylation of JNK and NF-kappaB p65 as well as reactive oxygen species (ROS) production by TNF-alpha. In vascular smooth muscle cells, eEF2K siRNA also inhibits VCAM-1 induction and phosphorylation of JNK and NF-kappaB by TNF-alpha. In vascular endothelial cells, siRNA against eEF2K inhibited induction of VCAM-1 and endothelial-selectin as well as monocyte adhesion by TNF-alpha. Phenotypes, overview Rattus norvegicus
2.7.11.20 physiological function eukaryotic elongation factor 2 kinase (eEF2K) prevents binding of eEF to the ribosome via phosphorylation of eEF and thus prevents further translation. The enzyme regulates the development of hypertension through oxidative stress-dependent vascular inflammation Homo sapiens
2.7.11.20 physiological function eukaryotic elongation factor 2 kinase (eEF2K) prevents binding of eEF to the ribosome via phosphorylation of eEF and thus prevents further translation. The enzyme regulates the development of hypertension through oxidative stress-dependent vascular inflammation. eEF2K protein increases in mesenteric artery from spontaneously hypertensive rats. Enzyme eEF2K mediates TNF-alpha-induced vascular inflammation via reactive oxygen species-dependent mechanism, which is at least partly responsible for the development of hypertension in spontaneously hypertensive rats Rattus norvegicus