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Literature summary extracted from

  • Bertwistle, D.; Vogt, L.; Aamudalapalli, H.B.; Palmer, D.R.; Sanders, D.A.
    Purification, crystallization and room-temperature X-ray diffraction of inositol dehydrogenase LcIDH2 from Lactobacillus casei BL23 (2014), Acta Crystallogr. Sect. F, 70, 979-983.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.18 gene iolG2, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain XL1-Blue Lacticaseibacillus casei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.18 purified recombinant His-tagged enzyme, microbatch crystallization, mixing of 0.001 ml of 10 mg/ml protein in 25 mM Tris-HCl, pH 8.0, with 0.001 ml of crystallization solution containing 2.8 M ammonium sulfate, 0.18 M citric acid, pH 5.0, room temperature, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement and model building, the room-temperature radiation tolerance of a protein crystal and its pseudo-translation symmetry, overview Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.18 38330
-
-
 Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.18 myo-inositol + NAD+ Lacticaseibacillus casei
-
 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
-
 ?
1.1.1.18 myo-inositol + NAD+ Lacticaseibacillus casei
-
 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ i.e. scyllo-inosose ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.18 Lacticaseibacillus casei E1U887 gene iolG1
-
1.1.1.18 Lacticaseibacillus casei E1U888 gene iolG2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.18 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XL1-Blue by ammonium sulfate fractionation, nickel affinity chromatography and dialysis Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.18 myo-inositol + NAD+
-
 Lacticaseibacillus casei 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
-
 ?
1.1.1.18 myo-inositol + NAD+
-
 Lacticaseibacillus casei 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ i.e. scyllo-inosose ?

Subunits

EC Number Subunits Comment Organism
1.1.1.18 tetramer 4 * 38330, sequence calculation and crystal structure analysis Lacticaseibacillus casei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.18 inositol dehydrogenase
-
 Lacticaseibacillus casei
1.1.1.18 iolG1
-
 Lacticaseibacillus casei
1.1.1.18 iolG2
-
 Lacticaseibacillus casei
1.1.1.18 LcIDH1
-
 Lacticaseibacillus casei
1.1.1.18 LcIDH2
-
 Lacticaseibacillus casei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.18 NAD+
-
 Lacticaseibacillus casei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.18 Lacticaseibacillus casei sequence calculation
-
 5.33