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Literature summary extracted from

  • Kim, E.; Kim, K.
    Cloning, expression, purification, crystallization and X-ray crystallographic analysis of (S)-3-hydroxybutyryl-CoA dehydrogenase from Clostridium butyricum (2014), Acta Crystallogr. Sect. F, 70, 485-488.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.35 recombinant expression of His6-tagged enzyme in Escherichia coli strain B834 Clostridium butyricum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.35 hanging drop vapour diffusion method, mixing of 30 mg/ml protein in 40 mM Tris-HCl, pH 8.0, 1 mM DTT, with reservoir solution containing 2 M ammonium sulfate, 0.1 M CAPS, pH 10.5, and 0.2 M lithium sulfate, 22°C, 7 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method and structure modeling Clostridium butyricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.35 30000
-
-
 Clostridium butyricum
1.1.1.35 60000
-
 recombinant His6-tagged enzyme, gel filtration Clostridium butyricum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutanoyl-CoA + NAD+ Clostridium butyricum
-
 acetoacetyl-CoA + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Clostridium butyricum C4IEM5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 recombinant His6-tagged enzyme from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration Clostridium butyricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 Clostridium butyricum acetoacetyl-CoA + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.35 homodimer 2 * 30000, recombinant His6-tagged enzyme, SDS-PAGE Clostridium butyricum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.35 (S)-3-hydroxybutyryl-CoA dehydrogenase
-
 Clostridium butyricum
1.1.1.35 CbHBD
-
 Clostridium butyricum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Clostridium butyricum
1.1.1.35 NADH
-
 Clostridium butyricum

General Information

EC Number General Information Comment Organism
1.1.1.35 metabolism the enzyme catalyzes the second step in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA Clostridium butyricum