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Literature summary extracted from

  • Yeo, K.J.; Han, Y.H.; Eo, Y.; Cheong, H.K.
    Expression, purification, crystallization and preliminary X-ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor (2013), Acta Crystallogr. Sect. F, 69, 909-911.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 cloning of the extracellular sensory domain of DraK, consisting of 88 residues (Glu28-Arg115) from genomic DNA, and overexpression as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.13.3 purified recombinant N-terminally His6-tagged extracellular sensory domain of DraK, sitting drop vapour diffusion method, mixing 200 nl of of 12 mg/ml protein in 10 mM sodium acetate, pH 4.5, and 100 mM NaCl with reservoir solution containing 0.2 M potassium/sodium tartrate, and 20% PEG 3350, and equilibration against 0.070 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement Streptomyces coelicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.13.3 membrane transmembrane protein Streptomyces coelicolor 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Streptomyces coelicolor Q9KZ83
-
-
2.7.13.3 Streptomyces coelicolor ATCC BAA-471D Q9KZ83
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 recombinant N-terminally His6-tagged extracellular sensory domain of DraK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Streptomyces coelicolor

Subunits

EC Number Subunits Comment Organism
2.7.13.3 More enzyme DraK consists of an extracellular domain flanked by two transmembrane helices (Leu5-Val27 and Leu126-Ile145), a sensory domain (Glu28-Thr125), a HAMP (histidine kinases, adenylyl cyclases, methyl-accepting chemotaxis proteins and phosphatases) domain (Asn149-Thr201) and a kinase domain (Ala202-Pro410) composed of His KA and CA domains Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 Drak
-
 Streptomyces coelicolor
2.7.13.3 DraK histidine kinase
-
 Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
2.7.13.3 physiological function DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in Streptomyces coelicolor through the DraR/DraK two-component system Streptomyces coelicolor