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Literature summary extracted from

  • Xu, Y.; Sun, F.
    Purification, crystallization and preliminary crystallographic analysis of 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans (2013), Acta Crystallogr. Sect. F, 69, 515-519.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.35 gene F54C8.1, recombinant expression of C-terminally GST-tagged enzyme in Escherichia coli strain BL21(DE3) Caenorhabditis elegans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.35 purified recombinant detagged enzyme, hanging drop vapour diffusion method, 10 mg/ml protein in 25 mM sodium acetate trihydrate, pH 5.0, and 300 mM sodium chloride is mixed with an equal volume of reservoir solution, containing 21% PEG 3350, 0.2 M sodium chloride, 0.1 M MES, pH 6.5, for parallelepiped-shaped crystals and 23% PEG 3350, 0.2 M sodium chloride, 0.1 M bicine, pH 8.0, for cuboid-shaped crystals, equilibration against 0.20 ml reservoir solution, 3-5days at 18°C, X-ray diffraction structure determination and analysis of parallelepiped-shaped cuboid-shaped crystal at 1.60 A and 2.20 A resolution, respectively Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.35 mitochondrial matrix
-
 Caenorhabditis elegans 5759
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxyacyl-CoA + NAD+ Caenorhabditis elegans
-
 3-oxoacyl-CoA + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Caenorhabditis elegans P34439
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 recombinant C-terminally GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, cleavage of the GST-tag by PPase, followed by gel filtration, anion exchange chromatography, and ultrafiltration Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxyacyl-CoA + NAD+
-
 Caenorhabditis elegans 3-oxoacyl-CoA + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.35 F54C8.1
-
 Caenorhabditis elegans
1.1.1.35 HAD
-
 Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Caenorhabditis elegans
1.1.1.35 NADH
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
1.1.1.35 evolution based on the chain length of the substrates, the HAD family is divided into three subclasses: 3-hydroxyacyl-CoA dehydrogenases (HADs), long-chain 3-hydroxyacyl-CoA dehydrogenases (LCHADs) and short-chain 3-hydroxyacyl-CoA dehydrogenases (SCHADs). HADs are soluble dimeric enzymes that exhibit substrate specificity for an acyl-chain length of C4-C10 and are previously referred to as short-chain HADs Caenorhabditis elegans
1.1.1.35 metabolism 3-hydroxyacyl-CoA dehydrogenase catalyzes the third step in fatty acid beta-oxidation, oxidizing the hydroxyl group of 3-hydroxyacyl-CoA to a keto group Caenorhabditis elegans