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Literature summary extracted from

  • Goyal, A.; Yousuf, M.; Rajakumara, E.; Arora, P.; Gokhale, R.; Sankaranarayanan, R.
    Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis (2006), Acta Crystallogr. Sect. F, 62, 350-352.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.49 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.49 hanging drop vapor diffusion method, using 20.3% (w/v) polyethylene glycol 3350, 0.1 M Na MES pH 6.0, 5 mM 2-mercaptoethanol and 0.13 M lithium sulfate Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.49 ATP + a long-chain fatty acid + holo-[mycocerosate synthase] Mycobacterium tuberculosis
-
 AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
 ?
6.2.1.49 ATP + a long-chain fatty acid + holo-[mycocerosate synthase] Mycobacterium tuberculosis H37Rv
-
 AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.49 Mycobacterium tuberculosis P9WQ59
-
-
6.2.1.49 Mycobacterium tuberculosis H37Rv P9WQ59
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.49 Ni-NTA column chromatography and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.49 ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
-
 Mycobacterium tuberculosis AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
 ?
6.2.1.49 ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
-
 Mycobacterium tuberculosis H37Rv AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.49 fadD28
-
 Mycobacterium tuberculosis