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Literature summary extracted from

  • Hicks, K.A.; Ealick, S.E.
    Biochemical and structural characterization of Klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway (2016), Acta Crystallogr. Sect. D, 72, 808-816.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.126 expression in Escherichia coli Klebsiella pneumoniae subsp. pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.126 to 2.9 A resolution. This active site is located in a solvent-exposed cleft with nonpolar amino acids lining the binding cleft, including Met423, Gly424 and Gly425. Other amino acids in the active site are Thr342, Ser360, Tyr362 and Leu405 Klebsiella pneumoniae subsp. pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.126 S360A mutant is able to autoprocess, but is catalytically inactive Klebsiella pneumoniae subsp. pneumoniae
3.5.1.126 T342A mutant does not autoprocess into a mature heterodimer and is inactive Klebsiella pneumoniae subsp. pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.126 20000
-
-
 Klebsiella pneumoniae subsp. pneumoniae
3.5.1.126 55000
-
-
 Klebsiella pneumoniae subsp. pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.126 oxamate + H2O Klebsiella pneumoniae subsp. pneumoniae
-
 oxalate + ammonia
-
 ?
3.5.1.126 oxamate + H2O Klebsiella pneumoniae subsp. pneumoniae ATCC 700721
-
 oxalate + ammonia
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.126 Klebsiella pneumoniae subsp. pneumoniae A6T9C8
-
-
3.5.1.126 Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 A6T9C8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.126 proteolytic modification protein autoprocessed from an inactive precursor to form a heterodimer, resulting in a 35.5 kDa alpha subunit and a 20 kDa beta subunit Klebsiella pneumoniae subsp. pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.126 oxamate + H2O
-
 Klebsiella pneumoniae subsp. pneumoniae oxalate + ammonia
-
 ?
3.5.1.126 oxamate + H2O
-
 Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 oxalate + ammonia
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.126 ? x * 55000, precursor protein, x *35500, alpha subunit, x * 20000, beta subunit, SDS-PAGE Klebsiella pneumoniae subsp. pneumoniae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.126 HpxW
-
 Klebsiella pneumoniae subsp. pneumoniae
3.5.1.126 KPN_01768
-
 Klebsiella pneumoniae subsp. pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.126 5.5
-
 oxamate pH 8.5, 22°C Klebsiella pneumoniae subsp. pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.126 1.159
-
 oxamate pH 8.5, 22°C Klebsiella pneumoniae subsp. pneumoniae