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Literature summary extracted from

  • Pampa, K.J.; Lokanath, N.K.; Kunishima, N.; Rai, R.V.
    The first crystal structure of NAD-dependent 3-dehydro-2-deoxy-D-gluconate dehydrogenase from Thermus thermophilus HB8 (2014), Acta Crystallogr. Sect. D, 70, 994-1004.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.126 in complex with NAD+, sitting drop vapor diffusion method, using 0.1 M bicine pH 9.0, 2% (w/v) dioxane, 10% (w/v) PEG 20 000 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.126 2-dehydro-3-deoxy-D-gluconate + NAD+ Thermus thermophilus
-
 (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
 ?
1.1.1.126 2-dehydro-3-deoxy-D-gluconate + NAD+ Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.126 Thermus thermophilus
-
-
-
1.1.1.126 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.126 Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT20-I column chromatography, and Superdex 75 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.126 2-dehydro-3-deoxy-D-gluconate + NAD+
-
 Thermus thermophilus (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
 ?
1.1.1.126 2-dehydro-3-deoxy-D-gluconate + NAD+
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.126 homotetramer X-ay crystallography Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.126 DDGDH
-
 Thermus thermophilus
1.1.1.126 NAD-dependent 3-dehydro-2-deoxy-D-gluconate dehydrogenase
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.126 NAD+ this enzyme prefers NAD+ as the physiological cofactor Thermus thermophilus