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Literature summary extracted from

  • Diez, V.; Loznik, M.; Taylor, S.; Winn, M.; Rattray, N.J.; Podmore, H.; Micklefield, J.; Goodacre, R.; Medema, M.H.; Mueller, U.; Bovenberg, R.; Janssen, D.B.; Takano, E.
    Functional exchangeability of oxidase and dehydrogenase reactions in the biosynthesis of hydroxyphenylglycine, a nonribosomal peptide building block (2015), ACS Synth. Biol., 4, 796-807.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.46 44
-
L-Mandelate at pH 7.5 and 28°C Streptomyces coelicolor
1.1.3.46 64
-
(S)-4-hydroxymandelate at pH 7.5 and 28°C Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.46 38200
-
x * 38200, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.46 (S)-4-hydroxymandelate + O2 Streptomyces coelicolor
-
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.46 Streptomyces coelicolor Q9Z4X8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.46 (S)-4-hydroxymandelate + O2
-
Streptomyces coelicolor 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
1.1.3.46 L-mandelate + O2
-
Streptomyces coelicolor ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.3.46 ? x * 38200, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.1.3.46 HmO
-
Streptomyces coelicolor
1.1.3.46 hydroxymandelate oxidase
-
Streptomyces coelicolor
1.1.3.46 Sco3228 locus name Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.46 1.2
-
L-Mandelate at pH 7.5 and 28°C Streptomyces coelicolor
1.1.3.46 3.5
-
(S)-4-hydroxymandelate at pH 7.5 and 28°C Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.46 FMN
-
Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.46 26.3
-
L-Mandelate at pH 7.5 and 28°C Streptomyces coelicolor
1.1.3.46 55.5
-
(S)-4-hydroxymandelate at pH 7.5 and 28°C Streptomyces coelicolor