BRENDA - Enzyme Database

The Escherichia coli periplasmic aldehyde oxidoreductase is an exceptional member of the xanthine oxidase family of molybdoenzymes

Correia, M.A.; Otrelo-Cardoso, A.R.; Schwuchow, V.; Sigfridsson Clauss, K.G.; Haumann, M.; Romao, M.J.; Leimkuehler, S.; Santos-Silva, T.; ACS Chem. Biol. 11, 2923-2935 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.17.1.4
-
Escherichia coli
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.17.1.4
to 1.7 A resolution. The active site of PaoABC is highly exposed to the surface with no aromatic residues and an arginine (subunit PaoC-R440) making a direct interaction with subunit PaoC-E692, which acts as a base catalyst
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.17.1.4
R40K
mutation in subunit PaoC, strong decrease in activity
Escherichia coli
1.17.1.4
R440H
mutation in subunit PaoC, strong decrease in activity, crystallization data
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.06
-
benzaldehyde
mutant R440H, pH 5.5, 22C
Escherichia coli
1.17.1.4
0.129
-
benzaldehyde
wild-type, pH 5.5, 22C
Escherichia coli
1.17.1.4
0.159
-
benzaldehyde
wild-type, pH 5.0, 22C
Escherichia coli
1.17.1.4
0.204
-
benzaldehyde
mutant R440K, pH 5.5, 22C
Escherichia coli
1.17.1.4
0.288
-
benzaldehyde
mutant R440H, pH 5.0, 22C
Escherichia coli
1.17.1.4
0.307
-
benzaldehyde
wild-type, pH 4.5, 22C
Escherichia coli
1.17.1.4
0.361
-
benzaldehyde
mutant R440K, pH 5.0, 22C
Escherichia coli
1.17.1.4
0.396
-
benzaldehyde
mutant R440H, pH 4.5, 22C
Escherichia coli
1.17.1.4
0.514
-
benzaldehyde
mutant R440H, pH 4.0, 22C
Escherichia coli
1.17.1.4
0.52
-
benzaldehyde
mutant R440K, pH 4.5, 22C
Escherichia coli
1.17.1.4
0.659
-
benzaldehyde
wild-type, pH 4.0, 22C
Escherichia coli
1.17.1.4
1.517
-
benzaldehyde
mutant R440K, pH 4.0, 22C
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Molybdenum
molybdopterin-cytosine-dinucleotide localized in subunit PaoC
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.4
Escherichia coli
P77165 and P77324 and P77489
P77165 i.e. alpha-subunit, P77324 i.e. beta-subnit, P77489 i.e. gamma-subunit
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
benzaldehyde + ferricyanide + H2O
-
739772
Escherichia coli
benzoate + 2 ferrocyanide + 2 H+
-
-
-
?
1.17.1.4
cinnamaldehyde + H2O + acceptor
-
739772
Escherichia coli
cinnamic acid + reduced acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.4
heterotrimer
crystallization data
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.17.1.4
41
-
benzaldehyde
mutant R440K, pH 5.5, 22C
Escherichia coli
1.17.1.4
48.7
-
benzaldehyde
mutant R440H, pH 5.5, 22C
Escherichia coli
1.17.1.4
80.5
-
benzaldehyde
mutant R440K, pH 5.0, 22C
Escherichia coli
1.17.1.4
150
-
benzaldehyde
mutant R440H, pH 5.0, 22C; wild-type, pH 5.5, 22C
Escherichia coli
1.17.1.4
217
-
benzaldehyde
wild-type, pH 5.0, 22C
Escherichia coli
1.17.1.4
293
-
benzaldehyde
mutant R440H, pH 4.5, 22C
Escherichia coli
1.17.1.4
307
-
benzaldehyde
mutant R440K, pH 4.0, 22C
Escherichia coli
1.17.1.4
325
-
benzaldehyde
mutant R440K, pH 4.5, 22C
Escherichia coli
1.17.1.4
404
-
benzaldehyde
mutant R440H, pH 4.0, 22C
Escherichia coli
1.17.1.4
486
-
benzaldehyde
wild-type, pH 4.5, 22C
Escherichia coli
1.17.1.4
1097
-
benzaldehyde
wild-type, pH 4.0, 22C
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
subunit PaoB exhibits a typical FAD binding motif
Escherichia coli
1.17.1.4
molybdopterin cytosine dinucleotide
localized in subunit PaoC
Escherichia coli
1.17.1.4
[2Fe-2S]-center
iron-sulfur subunit PaoA can be divided into two subdomains, each carrying one [2Fe-2S] cluster
Escherichia coli
1.17.1.4
[4Fe-4S]-center
cluster is anchored to an additional 40 residues subdomain of subunit PaoB
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.4
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
subunit PaoB exhibits a typical FAD binding motif
Escherichia coli
1.17.1.4
molybdopterin cytosine dinucleotide
localized in subunit PaoC
Escherichia coli
1.17.1.4
[2Fe-2S]-center
iron-sulfur subunit PaoA can be divided into two subdomains, each carrying one [2Fe-2S] cluster
Escherichia coli
1.17.1.4
[4Fe-4S]-center
cluster is anchored to an additional 40 residues subdomain of subunit PaoB
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.17.1.4
to 1.7 A resolution. The active site of PaoABC is highly exposed to the surface with no aromatic residues and an arginine (subunit PaoC-R440) making a direct interaction with subunit PaoC-E692, which acts as a base catalyst
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.17.1.4
R40K
mutation in subunit PaoC, strong decrease in activity
Escherichia coli
1.17.1.4
R440H
mutation in subunit PaoC, strong decrease in activity, crystallization data
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.06
-
benzaldehyde
mutant R440H, pH 5.5, 22C
Escherichia coli
1.17.1.4
0.129
-
benzaldehyde
wild-type, pH 5.5, 22C
Escherichia coli
1.17.1.4
0.159
-
benzaldehyde
wild-type, pH 5.0, 22C
Escherichia coli
1.17.1.4
0.204
-
benzaldehyde
mutant R440K, pH 5.5, 22C
Escherichia coli
1.17.1.4
0.288
-
benzaldehyde
mutant R440H, pH 5.0, 22C
Escherichia coli
1.17.1.4
0.307
-
benzaldehyde
wild-type, pH 4.5, 22C
Escherichia coli
1.17.1.4
0.361
-
benzaldehyde
mutant R440K, pH 5.0, 22C
Escherichia coli
1.17.1.4
0.396
-
benzaldehyde
mutant R440H, pH 4.5, 22C
Escherichia coli
1.17.1.4
0.514
-
benzaldehyde
mutant R440H, pH 4.0, 22C
Escherichia coli
1.17.1.4
0.52
-
benzaldehyde
mutant R440K, pH 4.5, 22C
Escherichia coli
1.17.1.4
0.659
-
benzaldehyde
wild-type, pH 4.0, 22C
Escherichia coli
1.17.1.4
1.517
-
benzaldehyde
mutant R440K, pH 4.0, 22C
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Molybdenum
molybdopterin-cytosine-dinucleotide localized in subunit PaoC
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
benzaldehyde + ferricyanide + H2O
-
739772
Escherichia coli
benzoate + 2 ferrocyanide + 2 H+
-
-
-
?
1.17.1.4
cinnamaldehyde + H2O + acceptor
-
739772
Escherichia coli
cinnamic acid + reduced acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.4
heterotrimer
crystallization data
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.17.1.4
41
-
benzaldehyde
mutant R440K, pH 5.5, 22C
Escherichia coli
1.17.1.4
48.7
-
benzaldehyde
mutant R440H, pH 5.5, 22C
Escherichia coli
1.17.1.4
80.5
-
benzaldehyde
mutant R440K, pH 5.0, 22C
Escherichia coli
1.17.1.4
150
-
benzaldehyde
mutant R440H, pH 5.0, 22C; wild-type, pH 5.5, 22C
Escherichia coli
1.17.1.4
217
-
benzaldehyde
wild-type, pH 5.0, 22C
Escherichia coli
1.17.1.4
293
-
benzaldehyde
mutant R440H, pH 4.5, 22C
Escherichia coli
1.17.1.4
307
-
benzaldehyde
mutant R440K, pH 4.0, 22C
Escherichia coli
1.17.1.4
325
-
benzaldehyde
mutant R440K, pH 4.5, 22C
Escherichia coli
1.17.1.4
404
-
benzaldehyde
mutant R440H, pH 4.0, 22C
Escherichia coli
1.17.1.4
486
-
benzaldehyde
wild-type, pH 4.5, 22C
Escherichia coli
1.17.1.4
1097
-
benzaldehyde
wild-type, pH 4.0, 22C
Escherichia coli
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.17.1.4
200
-
benzaldehyde
mutant R440K, pH 4.0, 22C; mutant R440K, pH 5.5, 22C
Escherichia coli
1.17.1.4
220
-
benzaldehyde
mutant R440K, pH 5.0, 22C
Escherichia coli
1.17.1.4
520
-
benzaldehyde
mutant R440H, pH 5.0, 22C
Escherichia coli
1.17.1.4
620
-
benzaldehyde
mutant R440K, pH 4.5, 22C
Escherichia coli
1.17.1.4
740
-
benzaldehyde
mutant R440H, pH 4.5, 22C
Escherichia coli
1.17.1.4
790
-
benzaldehyde
mutant R440H, pH 4.0, 22C
Escherichia coli
1.17.1.4
810
-
benzaldehyde
mutant R440H, pH 5.5, 22C
Escherichia coli
1.17.1.4
1170
-
benzaldehyde
wild-type, pH 5.5, 22C
Escherichia coli
1.17.1.4
1370
-
benzaldehyde
wild-type, pH 5.0, 22C
Escherichia coli
1.17.1.4
1580
-
benzaldehyde
wild-type, pH 4.5, 22C
Escherichia coli
1.17.1.4
1660
-
benzaldehyde
wild-type, pH 4.0, 22C
Escherichia coli
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.17.1.4
200
-
benzaldehyde
mutant R440K, pH 4.0, 22C; mutant R440K, pH 5.5, 22C
Escherichia coli
1.17.1.4
220
-
benzaldehyde
mutant R440K, pH 5.0, 22C
Escherichia coli
1.17.1.4
520
-
benzaldehyde
mutant R440H, pH 5.0, 22C
Escherichia coli
1.17.1.4
620
-
benzaldehyde
mutant R440K, pH 4.5, 22C
Escherichia coli
1.17.1.4
740
-
benzaldehyde
mutant R440H, pH 4.5, 22C
Escherichia coli
1.17.1.4
790
-
benzaldehyde
mutant R440H, pH 4.0, 22C
Escherichia coli
1.17.1.4
810
-
benzaldehyde
mutant R440H, pH 5.5, 22C
Escherichia coli
1.17.1.4
1170
-
benzaldehyde
wild-type, pH 5.5, 22C
Escherichia coli
1.17.1.4
1370
-
benzaldehyde
wild-type, pH 5.0, 22C
Escherichia coli
1.17.1.4
1580
-
benzaldehyde
wild-type, pH 4.5, 22C
Escherichia coli
1.17.1.4
1660
-
benzaldehyde
wild-type, pH 4.0, 22C
Escherichia coli