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Literature summary extracted from

  • Jain, S.; Afley, P.; Kumar, S.
    Immunological responses to recombinant cysteine synthase A of Brucella abortus in BALB/c mice (2013), World J. Microbiol. Biotechnol., 29, 907-913.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.47 medicine purified recombinant CysK is usefula as vaccine against Brucella in BALB/c mice, maximum protection by usage of rCysK-Al with aluminium hydroxide gel formulation followed by Freund's adjuvant formulation Brucella abortus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 gene cysK, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide Brucella abortus
-
 L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Brucella abortus C4ITG6 several strains, gene cysK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity and over 95% purity Brucella abortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide
-
 Brucella abortus L-cysteine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 cysK
-
 Brucella abortus
2.5.1.47 cysteine synthase A
-
 Brucella abortus