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Literature summary extracted from

  • Urakova, N.; Netzler, N.; Kelly, A.G.; Frese, M.; White, P.A.; Strive, T.
    Purification and biochemical characterisation of rabbit calicivirus RNA-dependent RNA polymerases and identification of non-nucleoside inhibitors (2016), Viruses, 8, 100.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.48 drug development the virus causes significant economic losses in rabbit farming and reduces wild rabbit populations, and its RNA-dependent RNA polymerase (RdRp) is a primary target for the development of antiviral drugs Rabbit hemorrhagic disease virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli Rabbit hemorrhagic disease virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 (4E)-1-(3,4-dimethylphenyl)-4-(hydroxyimino)pyrazolidine-3,5-dione
-
 Rabbit hemorrhagic disease virus
2.7.7.48 5-phenyl-2H-1,2,3-triazole-4-carboxylic acid
-
 Rabbit hemorrhagic disease virus
2.7.7.48 EDTA
-
 Rabbit hemorrhagic disease virus
2.7.7.48 N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-methyl-2-phenyl-1,3-thiazole-5-carboxamide a non-nucleoside inhibitor Rabbit hemorrhagic disease virus
2.7.7.48 rGTP
-
 Rabbit hemorrhagic disease virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 additional information
-
 additional information Michaelis-Menten kinetic analysis Rabbit hemorrhagic disease virus
2.7.7.48 14.6
-
 rGTP Michaelis-Menten kinetic model, pH 7.5, 30°C, recombinant enzyme Rabbit hemorrhagic disease virus
2.7.7.48 19.1
-
 rGTP substrate inhibition, pH 7.5, 30°C, recombinant enzyme Rabbit hemorrhagic disease virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 additional information Rabbit hemorrhagic disease virus RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity ?
-
 ?
2.7.7.48 additional information Rabbit hemorrhagic disease virus V-351 RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity ?
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn Rabbit hemorrhagic disease virus
-
 diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn Rabbit hemorrhagic disease virus V-351
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Rabbit hemorrhagic disease virus P27411 polyprotein; a calicivirus
-
2.7.7.48 Rabbit hemorrhagic disease virus V-351 P27411 polyprotein; a calicivirus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 recombinant C-terminally HIs6-tagged enzyme from Escherichia coli by nickel affinity chromatography Rabbit hemorrhagic disease virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.48 additional information
-
 the in vitro polymerase activity of a non-pathogenic calicivirus RdRp is at least two times higher than that of the RdRp of the highly virulent RHDV Rabbit hemorrhagic disease virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 additional information RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity Rabbit hemorrhagic disease virus ?
-
 ?
2.7.7.48 additional information RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity Rabbit hemorrhagic disease virus V-351 ?
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn
-
 Rabbit hemorrhagic disease virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn
-
 Rabbit hemorrhagic disease virus V-351 diphosphate + RNAn+1
-
 ?
2.7.7.48 rGTP + RNAn
-
 Rabbit hemorrhagic disease virus diphosphate + RNAn+1
-
 ?
2.7.7.48 rGTP + RNAn
-
 Rabbit hemorrhagic disease virus V-351 diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 More crystal structure of RHDV RdRp reveals that the enzyme adopts a shape that resembles a right hand, with domains corresponding to the fingers, palm and thumb Rabbit hemorrhagic disease virus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RDRP
-
 Rabbit hemorrhagic disease virus
2.7.7.48 RNA-dependent RNA polymerase
-
 Rabbit hemorrhagic disease virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 40 45
-
 Rabbit hemorrhagic disease virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 8.5
-
-
 Rabbit hemorrhagic disease virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.48 2.1
-
 rGTP substrate inhibition, pH 7.5, 30°C, recombinant enzyme Rabbit hemorrhagic disease virus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.48 0.0199
-
 pH 7.5, 30°C, recombinant enzyme Rabbit hemorrhagic disease virus N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-methyl-2-phenyl-1,3-thiazole-5-carboxamide

General Information

EC Number General Information Comment Organism
2.7.7.48 additional information the in vitro polymerase activity of a non-pathogenic calicivirus RdRp is at least two times higher than that of the RdRp of the highly virulent RHDV Rabbit hemorrhagic disease virus