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Literature summary extracted from

  • Mancilla, M.; Grillo, M.J.; de Miguel, M.J.; Lopez-Goni, I.; San-Roman, B.; Zabalza-Barangua, A.; Moriyon, I.
    Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine (2013), Vet. Res., 44, 105.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.B22 additional information generation of strain Rev2, by Rev 1 double in-frame deletion mutation in ISBm-1 transposase and GI-2 phage integrase giving mutant strain Rev2, improves the stability of Brucella melitensis Rev 1 vaccine. The parental Rev 1 strain, the Rev 2 double mutant DELTAISBm1DELTAint strain, and the virulent Bacillus melitensis strain H38 (as a control) are inoculated Brucella melitensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B22 Brucella melitensis
-
-
-
2.7.7.B22 Brucella melitensis Rev1
-
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B22 IS711
-
 Brucella melitensis
2.7.7.B22 Rev 1 transposase
-
 Brucella melitensis
2.7.7.B22 Rev 2 transposase
-
 Brucella melitensis
2.7.7.B22 wbk IS transposase
-
 Brucella melitensis
2.7.7.B22 wbkA flanking transposase
-
 Brucella melitensis
2.7.7.B22 wbkA-flanking IS
-
 Brucella melitensis

General Information

EC Number General Information Comment Organism
2.7.7.B22 evolution the enzyme belongs to the IS711 transposases Brucella melitensis
2.7.7.B22 malfunction mutation of the GI-2 int gene and the wbk IS transposase in Rev 1, resulting in strain Rev2, maintains the S phenotype and shows lower dissociation levels. Combining the two mutations results in a strain (Rev 2) displaying a 95% decrease in dissociation with respect to parental Rev 1 under conditions promoting dissociation. Rev 2 does not differ from Rev 1 in the characteristics used in Rev 1 typing, i.e. growth rate, colonial size, reactivity with O-polysaccharide antibodies, phage, dye and antibiotic susceptibility. Strains Rev 2 and Rev 1 show similar attenuation and afforded similar protection in the mouse model of brucellosis vaccines. Deletions involving GI-2 and wbkA are the major causes of S-R dissociation of Bacillus melitensis strain Rev 1 Brucella melitensis
2.7.7.B22 additional information deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis , Rev 1 mutant strain Rev2 is more effectively used as a vaccine compared to strain Rev 1, overview Brucella melitensis