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Literature summary extracted from

  • Bhatia, S.; Bhakri, G.; Arora, M.; Batta, S.; Uppal, S.
    Kinetic and thermodynamic properties of partially purified dextranase from Paecilomyces lilacinus and its application in dextran removal from cane juice (2016), Sugar Tech., 18, 204-213.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 Ca2+ 5 mM, isoform D-I 71% residual activity, isoform D-II 83% residual activity Purpureocillium lilacinum
3.2.1.11 Fe2+ 5 mM, isoform D-I 47% residual activity, isoform D-II 38% residual activity Purpureocillium lilacinum
3.2.1.11 Mn2+ 5 mM, isoform D-I 67% residual activity, isoform D-II 63% residual activity Purpureocillium lilacinum
3.2.1.11 Pb2+ 5 mM, isoform D-I 23% residual activity, isoform D-II 10% residual activity Purpureocillium lilacinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.11 3.2
-
 Dextran isoform D-I, calculated as released reducing sugar, 40°C, pH 5.0 Purpureocillium lilacinum
3.2.1.11 3.3
-
 Dextran isoform D-II, calculated as released reducing sugar, 40°C, pH 5.0 Purpureocillium lilacinum
3.2.1.11 5.9
-
 Dextran isoform D-II, calculated as released reducing sugar, 40°C, pH 4.0 Purpureocillium lilacinum
3.2.1.11 7.1
-
 Dextran isoform D-I, calculated as released reducing sugar, 40°C, pH 6.0 Purpureocillium lilacinum
3.2.1.11 7.14
-
 Dextran isoform D-I, calculated as released reducing sugar, 40°C, pH 4.0 Purpureocillium lilacinum
3.2.1.11 8.3
-
 Dextran isoform D-II, calculated as released reducing sugar, 40°C, pH 3.0 Purpureocillium lilacinum
3.2.1.11 9.1
-
 Dextran isoform D-I, calculated as released reducing sugar, 40°C, pH 3.0 Purpureocillium lilacinum
3.2.1.11 9.1
-
 Dextran isoform D-II, calculated as released reducing sugar, 40°C, pH 6.0 Purpureocillium lilacinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.11 Cu2+ 5 mM, 125% of initial activity Purpureocillium lilacinum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Purpureocillium lilacinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11 partial Purpureocillium lilacinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Purpureocillium lilacinum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 50
-
 both isoform D-I and D-II Purpureocillium lilacinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.11
-
 50 stable Purpureocillium lilacinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.11 5
-
 both isoform D-I and D-II Purpureocillium lilacinum