Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, R.; Sen, N.; Paul, B.D.; Snowman, A.M.; Rao, F.; Vandiver, M.S.; Xu, J.; Snyder, S.H.
    Inositol polyphosphate multikinase is a coactivator of p53-mediated transcription and cell death (2013), Sci. Signal., 6, ra22.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.151 gene impk, recombinant GST-tagged IMPK overexpression in human colon cancer cell line HCT-116 and the human osteosarcoma cell line U2-OS. Overexpression of kinase-deficient IPMK inhibits cell proliferation in etoposide-treated U2OS cells to a similar extent as does wild-type IPMK Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.151 K129A/S235A catalytically inactive mutant Homo sapiens
2.7.1.151 additional information genetic or RNA interference-mediated, and shRNA-mediated knockdown of enzyme IPMK Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.151 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ?
2.7.1.151 additional information Homo sapiens IPMK is a broad-specificity enzyme that converts inositol 1,4,5-trisphosphate into inositol 1,4,5,6-tetrakisphosphate (IP4) and subsequently inositol 1,3,4,5,6-pentakisphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.151 Homo sapiens Q8NFU5 gene impk
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.151 HCT-116 cell
-
 Homo sapiens
-
2.7.1.151 U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ?
2.7.1.151 additional information IPMK is a broad-specificity enzyme that converts inositol 1,4,5-trisphosphate into inositol 1,4,5,6-tetrakisphosphate (IP4) and subsequently inositol 1,3,4,5,6-pentakisphosphate Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.151 inositol polyphosphate multikinase
-
 Homo sapiens
2.7.1.151 IPMK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.151 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.151 malfunction knockdown of IPMK results in decreased activation of p53, decreased recruitment of p53 and p300 to target gene promoters, abrogated transcription of p53 target genes, and enhanced cell viability. Blocking the IPMK-p53 interaction decreases the extent of p53-mediated transcription. Depletion of IPMK results in decreased PUMA, Bax, and p21 abundance after treatment with etoposide, p53-null HCT116 cells transfected with IPMK shRNA do not exhibit decreased amounts of PUMA, Bax, or p21 mRNAs. In etoposide-treated HCT-116 cells, shRNA-mediated knockdown of IPMK reduces the binding of p300 to p53 Homo sapiens
2.7.1.151 physiological function the enzyme stimulates tumor suppressor p53-mediated transcription by binding to p53 and enhancing its acetylation by the acetyltransferase p300 independently of its inositol phosphate and lipid kinase activities. IPMK acts as a transcriptional coactivator for p53 and that it is an integral part of the p53 transcriptional complex facilitating cell death. Tumor suppressor p53 is a critical transcriptional factor that senses and modulates cellular responses to injury and stress. Recombinantly expressed enzyme IPMK in the transfected cells binds to endogenous p53 upon treatment with etoposide, a DNA-damaging agent that canonically induces apoptosis by activating p53. IPMK enhances p53 acetylation and histone acetylation via p300, molecular mechanisms responsible for the stimulation of p53 transcriptional activity by IPMK, overview. IPMK does not require catalytic activity to enhance p53-mediated cell death Homo sapiens